Label-free optical quantification of structural alterations in Alzheimer’s disease

Label-free optical quantification of structural alterations in Alzheimer’s disease

We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light field images, scattering coefficients and anisotropies are quantitatively retrieved by usi...

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Main Authors: Lee, Moosung, Lee, Eeksung, Jung, JaeHwang, Yu, Hyeonseung, Kim, Kyoohyun, Yoon, Jonghee, Lee, Shinhwa, Jeong, Yong, Park, YongKeun
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971571/
id pubmed-4971571
recordtype oai_dc
spelling pubmed-49715712016-08-11 Label-free optical quantification of structural alterations in Alzheimer’s disease Lee, Moosung Lee, Eeksung Jung, JaeHwang Yu, Hyeonseung Kim, Kyoohyun Yoon, Jonghee Lee, Shinhwa Jeong, Yong Park, YongKeun Article We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light field images, scattering coefficients and anisotropies are quantitatively retrieved by using the modified the scattering-phase theorem, which enables access to structural information about brain tissues. As a proof of principle, we demonstrate that these scattering parameters enable us to quantitatively address structural alteration in the brain tissues of mice with Alzheimer’s disease. Nature Publishing Group 2016-08-03 /pmc/articles/PMC4971571/ /pubmed/27485313 http://dx.doi.org/10.1038/srep31034 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Lee, Moosung
Lee, Eeksung
Jung, JaeHwang
Yu, Hyeonseung
Kim, Kyoohyun
Yoon, Jonghee
Lee, Shinhwa
Jeong, Yong
Park, YongKeun
spellingShingle Lee, Moosung
Lee, Eeksung
Jung, JaeHwang
Yu, Hyeonseung
Kim, Kyoohyun
Yoon, Jonghee
Lee, Shinhwa
Jeong, Yong
Park, YongKeun
Label-free optical quantification of structural alterations in Alzheimer’s disease
author_facet Lee, Moosung
Lee, Eeksung
Jung, JaeHwang
Yu, Hyeonseung
Kim, Kyoohyun
Yoon, Jonghee
Lee, Shinhwa
Jeong, Yong
Park, YongKeun
author_sort Lee, Moosung
title Label-free optical quantification of structural alterations in Alzheimer’s disease
title_short Label-free optical quantification of structural alterations in Alzheimer’s disease
title_full Label-free optical quantification of structural alterations in Alzheimer’s disease
title_fullStr Label-free optical quantification of structural alterations in Alzheimer’s disease
title_full_unstemmed Label-free optical quantification of structural alterations in Alzheimer’s disease
title_sort label-free optical quantification of structural alterations in alzheimer’s disease
description We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light field images, scattering coefficients and anisotropies are quantitatively retrieved by using the modified the scattering-phase theorem, which enables access to structural information about brain tissues. As a proof of principle, we demonstrate that these scattering parameters enable us to quantitatively address structural alteration in the brain tissues of mice with Alzheimer’s disease.
publisher Nature Publishing Group
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971571/
_version_ 1613620378873102336

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