Serial femtosecond crystallography datasets from G protein-coupled receptors
We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: t...
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| Format: | Online |
| Language: | English |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968193/ |
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pubmed-4968193 |
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pubmed-49681932016-08-08 Serial femtosecond crystallography datasets from G protein-coupled receptors White, Thomas A. Barty, Anton Liu, Wei Ishchenko, Andrii Zhang, Haitao Gati, Cornelius Zatsepin, Nadia A. Basu, Shibom Oberthür, Dominik Metz, Markus Beyerlein, Kenneth R. Yoon, Chun Hong Yefanov, Oleksandr M. James, Daniel Wang, Dingjie Messerschmidt, Marc Koglin, Jason E. Boutet, Sébastien Weierstall, Uwe Cherezov, Vadim Data Descriptor We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data. Nature Publishing Group 2016-08-01 /pmc/articles/PMC4968193/ /pubmed/27479354 http://dx.doi.org/10.1038/sdata.2016.57 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0 Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
White, Thomas A. Barty, Anton Liu, Wei Ishchenko, Andrii Zhang, Haitao Gati, Cornelius Zatsepin, Nadia A. Basu, Shibom Oberthür, Dominik Metz, Markus Beyerlein, Kenneth R. Yoon, Chun Hong Yefanov, Oleksandr M. James, Daniel Wang, Dingjie Messerschmidt, Marc Koglin, Jason E. Boutet, Sébastien Weierstall, Uwe Cherezov, Vadim |
| spellingShingle |
White, Thomas A. Barty, Anton Liu, Wei Ishchenko, Andrii Zhang, Haitao Gati, Cornelius Zatsepin, Nadia A. Basu, Shibom Oberthür, Dominik Metz, Markus Beyerlein, Kenneth R. Yoon, Chun Hong Yefanov, Oleksandr M. James, Daniel Wang, Dingjie Messerschmidt, Marc Koglin, Jason E. Boutet, Sébastien Weierstall, Uwe Cherezov, Vadim Serial femtosecond crystallography datasets from G protein-coupled receptors |
| author_facet |
White, Thomas A. Barty, Anton Liu, Wei Ishchenko, Andrii Zhang, Haitao Gati, Cornelius Zatsepin, Nadia A. Basu, Shibom Oberthür, Dominik Metz, Markus Beyerlein, Kenneth R. Yoon, Chun Hong Yefanov, Oleksandr M. James, Daniel Wang, Dingjie Messerschmidt, Marc Koglin, Jason E. Boutet, Sébastien Weierstall, Uwe Cherezov, Vadim |
| author_sort |
White, Thomas A. |
| title |
Serial femtosecond crystallography datasets from G protein-coupled receptors |
| title_short |
Serial femtosecond crystallography datasets from G protein-coupled receptors |
| title_full |
Serial femtosecond crystallography datasets from G protein-coupled receptors |
| title_fullStr |
Serial femtosecond crystallography datasets from G protein-coupled receptors |
| title_full_unstemmed |
Serial femtosecond crystallography datasets from G protein-coupled receptors |
| title_sort |
serial femtosecond crystallography datasets from g protein-coupled receptors |
| description |
We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968193/ |
| _version_ |
1613618593320140800 |