PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins
Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...
Main Authors: | Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello |
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Format: | Online |
Language: | English |
Published: |
The Company of Biologists Ltd
2016
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958280/ |
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