PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Bibliographic Details
Main Authors: Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello
Format: Online
Language:English
Published: The Company of Biologists Ltd 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958280/