miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110

The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of ag...

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Main Authors:	Bijnsdorp, Irene V., Hodzic, Jasmina, Lagerweij, Tonny, Westerman, Bart, Krijgsman, Oscar, Broeke, Jurjen, Verweij, Frederik, Nilsson, R. Jonas A., Rozendaal, Lawrence, van Beusechem, Victor W., van Moorselaar, Jeroen A., Wurdinger, Thomas, Geldof, Albert A.
Format:	Online
Language:	English
Published:	Impact Journals LLC 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941343/

id	pubmed-4941343
recordtype	oai_dc
spelling	pubmed-49413432016-07-19 miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110 Bijnsdorp, Irene V. Hodzic, Jasmina Lagerweij, Tonny Westerman, Bart Krijgsman, Oscar Broeke, Jurjen Verweij, Frederik Nilsson, R. Jonas A. Rozendaal, Lawrence van Beusechem, Victor W. van Moorselaar, Jeroen A. Wurdinger, Thomas Geldof, Albert A. Research Paper The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of aggressive cell behaviour. However its regulation in metastatic cells remains unclear. Here we identified miR-129-3p as a novel metastatic microRNA. CP110 was identified as its target protein. In PCa cells that have metastatic capacity, CP110 expression was repressed by miR-129-3p. High miR-129-3p expression levels increased cell invasion, while increasing CP110 levels decreased cell invasion. Overexpression of CP110 in metastatic PCa cells resulted in a decrease in the number of metastasis. In tissues of PCa patients, low CP110 and high miR-129-3p expression levels correlated with metastasis, but not with the expression of genes related to EMT. Furthermore, overexpression of CP110 in metastatic PCa cells resulted in excessive-CA (E-CA), and a change in F-actin distribution which is in agreement with their reduced metastatic capacity. Our data demonstrate that miR-129-3p functions as a CA gatekeeper in metastatic PCa cells by maintaining pro-metastatic centrosome amplification (CA) and preventing anti-metastatic E-CA. Impact Journals LLC 2016-02-23 /pmc/articles/PMC4941343/ /pubmed/26918338 http://dx.doi.org/10.18632/oncotarget.7572 Text en Copyright: © 2016 Bijnsdorp et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bijnsdorp, Irene V. Hodzic, Jasmina Lagerweij, Tonny Westerman, Bart Krijgsman, Oscar Broeke, Jurjen Verweij, Frederik Nilsson, R. Jonas A. Rozendaal, Lawrence van Beusechem, Victor W. van Moorselaar, Jeroen A. Wurdinger, Thomas Geldof, Albert A.
spellingShingle	Bijnsdorp, Irene V. Hodzic, Jasmina Lagerweij, Tonny Westerman, Bart Krijgsman, Oscar Broeke, Jurjen Verweij, Frederik Nilsson, R. Jonas A. Rozendaal, Lawrence van Beusechem, Victor W. van Moorselaar, Jeroen A. Wurdinger, Thomas Geldof, Albert A. miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
author_facet	Bijnsdorp, Irene V. Hodzic, Jasmina Lagerweij, Tonny Westerman, Bart Krijgsman, Oscar Broeke, Jurjen Verweij, Frederik Nilsson, R. Jonas A. Rozendaal, Lawrence van Beusechem, Victor W. van Moorselaar, Jeroen A. Wurdinger, Thomas Geldof, Albert A.
author_sort	Bijnsdorp, Irene V.
title	miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
title_short	miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
title_full	miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
title_fullStr	miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
title_full_unstemmed	miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110
title_sort	mir-129-3p controls centrosome number in metastatic prostate cancer cells by repressing cp110
description	The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of aggressive cell behaviour. However its regulation in metastatic cells remains unclear. Here we identified miR-129-3p as a novel metastatic microRNA. CP110 was identified as its target protein. In PCa cells that have metastatic capacity, CP110 expression was repressed by miR-129-3p. High miR-129-3p expression levels increased cell invasion, while increasing CP110 levels decreased cell invasion. Overexpression of CP110 in metastatic PCa cells resulted in a decrease in the number of metastasis. In tissues of PCa patients, low CP110 and high miR-129-3p expression levels correlated with metastasis, but not with the expression of genes related to EMT. Furthermore, overexpression of CP110 in metastatic PCa cells resulted in excessive-CA (E-CA), and a change in F-actin distribution which is in agreement with their reduced metastatic capacity. Our data demonstrate that miR-129-3p functions as a CA gatekeeper in metastatic PCa cells by maintaining pro-metastatic centrosome amplification (CA) and preventing anti-metastatic E-CA.
publisher	Impact Journals LLC
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941343/
_version_	1613607691210457088

miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110

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