Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

We present a high content multiwell plate cell-based assay approach to quantify protein interactions directly in cells using Förster resonance energy transfer (FRET) read out by automated fluorescence lifetime imaging (FLIM). Automated FLIM is implemented using wide-field time-gated detection, typic...

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Bibliographic Details
Main Authors: Margineanu, Anca, Chan, Jia Jia, Kelly, Douglas J., Warren, Sean C., Flatters, Delphine, Kumar, Sunil, Katan, Matilda, Dunsby, Christopher W., French, Paul M. W.
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919659/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919659/

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