Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana

To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-based overexpression vector (TRBO) to express...

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Bibliographic Details
Main Authors: Alkanaimsh, Salem, Karuppanan, Kalimuthu, Guerrero, Andrés, Tu, Aye M., Hashimoto, Bryce, Hwang, Min Sook, Phu, My L., Arzola, Lucas, Lebrilla, Carlito B., Dandekar, Abhaya M., Falk, Bryce W., Nandi, Somen, Rodriguez, Raymond L., McDonald, Karen A.
Format: Online
Language:English
Published: Frontiers Media S.A. 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909763/