The TraDIS toolkit: sequencing and analysis for dense transposon mutant libraries

Summary: Transposon insertion sequencing is a high-throughput technique for assaying large libraries of otherwise isogenic transposon mutants providing insight into gene essentiality, gene function and genetic interactions. We previously developed the Transposon Directed Insertion Sequencing (TraDIS...

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Bibliographic Details
Main Authors: Barquist, Lars, Mayho, Matthew, Cummins, Carla, Cain, Amy K., Boinett, Christine J., Page, Andrew J., Langridge, Gemma C., Quail, Michael A., Keane, Jacqueline A., Parkhill, Julian
Format: Online
Language:English
Published: Oxford University Press 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4896371/