Dynamics of Translation of Single mRNA Molecules In Vivo
Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of...
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| Format: | Online |
| Language: | English |
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Cell Press
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889334/ |
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pubmed-48893342016-07-14 Dynamics of Translation of Single mRNA Molecules In Vivo Yan, Xiaowei Hoek, Tim A. Vale, Ronald D. Tanenbaum, Marvin E. Resource Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising heterogeneity in the translation of individual mRNAs within the same cell, including rapid and reversible transitions between a translating and non-translating state. Applying this method to the cell-cycle gene Emi1, we find strong overall repression of translation initiation by specific 5′ UTR sequences, but individual mRNA molecules in the same cell can exhibit dramatically different translational efficiencies. The ability to observe translation of single mRNA molecules in live cells provides a powerful tool to study translation regulation. Cell Press 2016-05-05 /pmc/articles/PMC4889334/ /pubmed/27153498 http://dx.doi.org/10.1016/j.cell.2016.04.034 Text en © 2016 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Yan, Xiaowei Hoek, Tim A. Vale, Ronald D. Tanenbaum, Marvin E. |
| spellingShingle |
Yan, Xiaowei Hoek, Tim A. Vale, Ronald D. Tanenbaum, Marvin E. Dynamics of Translation of Single mRNA Molecules In Vivo |
| author_facet |
Yan, Xiaowei Hoek, Tim A. Vale, Ronald D. Tanenbaum, Marvin E. |
| author_sort |
Yan, Xiaowei |
| title |
Dynamics of Translation of Single mRNA Molecules In Vivo |
| title_short |
Dynamics of Translation of Single mRNA Molecules In Vivo |
| title_full |
Dynamics of Translation of Single mRNA Molecules In Vivo |
| title_fullStr |
Dynamics of Translation of Single mRNA Molecules In Vivo |
| title_full_unstemmed |
Dynamics of Translation of Single mRNA Molecules In Vivo |
| title_sort |
dynamics of translation of single mrna molecules in vivo |
| description |
Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising heterogeneity in the translation of individual mRNAs within the same cell, including rapid and reversible transitions between a translating and non-translating state. Applying this method to the cell-cycle gene Emi1, we find strong overall repression of translation initiation by specific 5′ UTR sequences, but individual mRNA molecules in the same cell can exhibit dramatically different translational efficiencies. The ability to observe translation of single mRNA molecules in live cells provides a powerful tool to study translation regulation. |
| publisher |
Cell Press |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889334/ |
| _version_ |
1613587417927778304 |