Development of a quantitative fluorescence-based ligand-binding assay

A major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This li...

Full description

Bibliographic Details
Main Authors: Breen, Conor J., Raverdeau, Mathilde, Voorheis, H. Paul
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861924/