A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity
In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in posttranscriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease–exonuclease–...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford Journals
2013
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| Online Access: | http://eprints.nottingham.ac.uk/2323/ http://eprints.nottingham.ac.uk/2323/ http://eprints.nottingham.ac.uk/2323/ http://eprints.nottingham.ac.uk/2323/1/Winkler_Fluorescence.pdf |