Diverse human extracellular RNAs are widely detected in human plasma

There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing dat...

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Main Authors:	Freedman, Jane E., Gerstein, Mark, Mick, Eric, Rozowsky, Joel, Levy, Daniel, Kitchen, Robert, Das, Saumya, Shah, Ravi, Danielson, Kirsty, Beaulieu, Lea, Navarro, Fabio C. P., Wang, Yaoyu, Galeev, Timur R., Holman, Alex, Kwong, Raymond Y., Murthy, Venkatesh, Tanriverdi, Selim E., Koupenova, Milka, Mikhalev, Ekaterina, Tanriverdi, Kahraman
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853467/

id	pubmed-4853467
recordtype	oai_dc
spelling	pubmed-48534672016-05-10 Diverse human extracellular RNAs are widely detected in human plasma Freedman, Jane E. Gerstein, Mark Mick, Eric Rozowsky, Joel Levy, Daniel Kitchen, Robert Das, Saumya Shah, Ravi Danielson, Kirsty Beaulieu, Lea Navarro, Fabio C. P. Wang, Yaoyu Galeev, Timur R. Holman, Alex Kwong, Raymond Y. Murthy, Venkatesh Tanriverdi, Selim E. Koupenova, Milka Mikhalev, Ekaterina Tanriverdi, Kahraman Article There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population. Nature Publishing Group 2016-04-26 /pmc/articles/PMC4853467/ /pubmed/27112789 http://dx.doi.org/10.1038/ncomms11106 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Freedman, Jane E. Gerstein, Mark Mick, Eric Rozowsky, Joel Levy, Daniel Kitchen, Robert Das, Saumya Shah, Ravi Danielson, Kirsty Beaulieu, Lea Navarro, Fabio C. P. Wang, Yaoyu Galeev, Timur R. Holman, Alex Kwong, Raymond Y. Murthy, Venkatesh Tanriverdi, Selim E. Koupenova, Milka Mikhalev, Ekaterina Tanriverdi, Kahraman
spellingShingle	Freedman, Jane E. Gerstein, Mark Mick, Eric Rozowsky, Joel Levy, Daniel Kitchen, Robert Das, Saumya Shah, Ravi Danielson, Kirsty Beaulieu, Lea Navarro, Fabio C. P. Wang, Yaoyu Galeev, Timur R. Holman, Alex Kwong, Raymond Y. Murthy, Venkatesh Tanriverdi, Selim E. Koupenova, Milka Mikhalev, Ekaterina Tanriverdi, Kahraman Diverse human extracellular RNAs are widely detected in human plasma
author_facet	Freedman, Jane E. Gerstein, Mark Mick, Eric Rozowsky, Joel Levy, Daniel Kitchen, Robert Das, Saumya Shah, Ravi Danielson, Kirsty Beaulieu, Lea Navarro, Fabio C. P. Wang, Yaoyu Galeev, Timur R. Holman, Alex Kwong, Raymond Y. Murthy, Venkatesh Tanriverdi, Selim E. Koupenova, Milka Mikhalev, Ekaterina Tanriverdi, Kahraman
author_sort	Freedman, Jane E.
title	Diverse human extracellular RNAs are widely detected in human plasma
title_short	Diverse human extracellular RNAs are widely detected in human plasma
title_full	Diverse human extracellular RNAs are widely detected in human plasma
title_fullStr	Diverse human extracellular RNAs are widely detected in human plasma
title_full_unstemmed	Diverse human extracellular RNAs are widely detected in human plasma
title_sort	diverse human extracellular rnas are widely detected in human plasma
description	There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853467/
_version_	1613574189461012480

Diverse human extracellular RNAs are widely detected in human plasma

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