Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo

Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo

Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundant...

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Main Authors: Gillet, Nicolas A., Hamaidia, Malik, de Brogniez, Alix, Gutiérrez, Gerónimo, Renotte, Nathalie, Reichert, Michal, Trono, Karina, Willems, Luc
Format: Online
Language:English
Published: Public Library of Science 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849745/
id pubmed-4849745
recordtype oai_dc
spelling pubmed-48497452016-05-07 Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo Gillet, Nicolas A. Hamaidia, Malik de Brogniez, Alix Gutiérrez, Gerónimo Renotte, Nathalie Reichert, Michal Trono, Karina Willems, Luc Research Article Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host. Public Library of Science 2016-04-28 /pmc/articles/PMC4849745/ /pubmed/27123579 http://dx.doi.org/10.1371/journal.ppat.1005588 Text en © 2016 Gillet et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Gillet, Nicolas A.
Hamaidia, Malik
de Brogniez, Alix
Gutiérrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina
Willems, Luc
spellingShingle Gillet, Nicolas A.
Hamaidia, Malik
de Brogniez, Alix
Gutiérrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina
Willems, Luc
Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
author_facet Gillet, Nicolas A.
Hamaidia, Malik
de Brogniez, Alix
Gutiérrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina
Willems, Luc
author_sort Gillet, Nicolas A.
title Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
title_short Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
title_full Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
title_fullStr Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
title_full_unstemmed Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo
title_sort bovine leukemia virus small noncoding rnas are functional elements that regulate replication and contribute to oncogenesis in vivo
description Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.
publisher Public Library of Science
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849745/
_version_ 1613572890920222720

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