Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells

In the current concept, tRNA maturation in vertebrate cells, including splicing of introns, trimming of 5’ leader and 3’ trailer, and adding of CCA, is thought to occur exclusively in the nucleus. Here we provide evidence to challenge this concept. Unspliced intron-containing precursor tRNAIle was i...

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Main Authors:	Wei, Min, Zhao, Xia, Liu, Mi, Niu, Meijuan, Seif, Elias, Kleiman, Lawrence
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839721/

id	pubmed-4839721
recordtype	oai_dc
spelling	pubmed-48397212016-04-29 Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells Wei, Min Zhao, Xia Liu, Mi Niu, Meijuan Seif, Elias Kleiman, Lawrence Research Article In the current concept, tRNA maturation in vertebrate cells, including splicing of introns, trimming of 5’ leader and 3’ trailer, and adding of CCA, is thought to occur exclusively in the nucleus. Here we provide evidence to challenge this concept. Unspliced intron-containing precursor tRNAIle was identified in Human Immunodeficiency Virus type 1 (HIV-1) virions, which are synthesized in the cytoplasm. Northern blot, confocal microscopy and quantitative RT-PCR further verified enrichment of this unspliced tRNAIle within the cytoplasm in human cells. In addition to containing an intron, the cytoplasmic precursor tRNAIle also contains a short incompletely processed 5´ leader and a 3´ trailer, which abundance is around 1000 fold higher than the nuclear precursor tRNAIle with long 5’ leader and long 3’ trailer. In vitro data also suggest that the cytoplasmic unspliced end-immature precursor tRNAIle could be processed by short isoform of RNase Z, but not long isoform of RNase Z. These data suggest that precursor tRNAs could export from the nucleus to the cytoplasm in human cells, instead of be processed only in the nucleus. Public Library of Science 2016-04-21 /pmc/articles/PMC4839721/ /pubmed/27101286 http://dx.doi.org/10.1371/journal.pone.0154044 Text en © 2016 Wei et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wei, Min Zhao, Xia Liu, Mi Niu, Meijuan Seif, Elias Kleiman, Lawrence
spellingShingle	Wei, Min Zhao, Xia Liu, Mi Niu, Meijuan Seif, Elias Kleiman, Lawrence Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
author_facet	Wei, Min Zhao, Xia Liu, Mi Niu, Meijuan Seif, Elias Kleiman, Lawrence
author_sort	Wei, Min
title	Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
title_short	Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
title_full	Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
title_fullStr	Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
title_full_unstemmed	Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells
title_sort	export of precursor trnaile from the nucleus to the cytoplasm in human cells
description	In the current concept, tRNA maturation in vertebrate cells, including splicing of introns, trimming of 5’ leader and 3’ trailer, and adding of CCA, is thought to occur exclusively in the nucleus. Here we provide evidence to challenge this concept. Unspliced intron-containing precursor tRNAIle was identified in Human Immunodeficiency Virus type 1 (HIV-1) virions, which are synthesized in the cytoplasm. Northern blot, confocal microscopy and quantitative RT-PCR further verified enrichment of this unspliced tRNAIle within the cytoplasm in human cells. In addition to containing an intron, the cytoplasmic precursor tRNAIle also contains a short incompletely processed 5´ leader and a 3´ trailer, which abundance is around 1000 fold higher than the nuclear precursor tRNAIle with long 5’ leader and long 3’ trailer. In vitro data also suggest that the cytoplasmic unspliced end-immature precursor tRNAIle could be processed by short isoform of RNase Z, but not long isoform of RNase Z. These data suggest that precursor tRNAs could export from the nucleus to the cytoplasm in human cells, instead of be processed only in the nucleus.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839721/
_version_	1613569091157622784

Export of Precursor tRNAIle from the Nucleus to the Cytoplasm in Human Cells

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