The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan

The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan

In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-r...

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Main Authors: IGUCHI, Aiko, SOMA, Takehisa, SUZUKI, Hiroshi, XUAN, Xuenan
Format: Online
Language:English
Published: The Japanese Society of Veterinary Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829523/
id pubmed-4829523
recordtype oai_dc
spelling pubmed-48295232016-04-13 The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan IGUCHI, Aiko SOMA, Takehisa SUZUKI, Hiroshi XUAN, Xuenan Internal Medicine In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals. The Japanese Society of Veterinary Science 2015-11-09 2016-03 /pmc/articles/PMC4829523/ /pubmed/26549436 http://dx.doi.org/10.1292/jvms.15-0480 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author IGUCHI, Aiko
SOMA, Takehisa
SUZUKI, Hiroshi
XUAN, Xuenan
spellingShingle IGUCHI, Aiko
SOMA, Takehisa
SUZUKI, Hiroshi
XUAN, Xuenan
The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
author_facet IGUCHI, Aiko
SOMA, Takehisa
SUZUKI, Hiroshi
XUAN, Xuenan
author_sort IGUCHI, Aiko
title The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
title_short The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
title_full The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
title_fullStr The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
title_full_unstemmed The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
title_sort epidemiological survey for atovaquone resistant related gene of babesia gibsoni in japan
description In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.
publisher The Japanese Society of Veterinary Science
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829523/
_version_ 1613565394418663424

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