Generation of Hprt-disrupted rat through mouse←rat ES chimeras
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and tra...
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| Format: | Online |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827079/ |
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pubmed-48270792016-04-19 Generation of Hprt-disrupted rat through mouse←rat ES chimeras Isotani, Ayako Yamagata, Kazuo Okabe, Masaru Ikawa, Masahito Article We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse←rat ES chimera system. The mouse←rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production. Nature Publishing Group 2016-04-11 /pmc/articles/PMC4827079/ /pubmed/27062982 http://dx.doi.org/10.1038/srep24215 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Isotani, Ayako Yamagata, Kazuo Okabe, Masaru Ikawa, Masahito |
| spellingShingle |
Isotani, Ayako Yamagata, Kazuo Okabe, Masaru Ikawa, Masahito Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| author_facet |
Isotani, Ayako Yamagata, Kazuo Okabe, Masaru Ikawa, Masahito |
| author_sort |
Isotani, Ayako |
| title |
Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| title_short |
Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| title_full |
Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| title_fullStr |
Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| title_full_unstemmed |
Generation of Hprt-disrupted rat through mouse←rat ES chimeras |
| title_sort |
generation of hprt-disrupted rat through mouse←rat es chimeras |
| description |
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse←rat ES chimera system. The mouse←rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827079/ |
| _version_ |
1613564547543597056 |