An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design

An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design

Precision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designe...

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Bibliographic Details
Main Authors: Zhao, Yongping, Zhang, Congsheng, Liu, Wenwen, Gao, Wei, Liu, Changlin, Song, Gaoyuan, Li, Wen-Xue, Mao, Long, Chen, Beijiu, Xu, Yunbi, Li, Xinhai, Xie, Chuanxiao
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817149/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817149/

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