nagZ Triggers Gonococcal Biofilm Disassembly

Bacterial-bacterial interactions play a critical role in promoting biofilm formation. Here we show that NagZ, a protein associated with peptidoglycan recycling, has moonlighting activity that allows it to modulate biofilm accumulation by Neisseria gonorrhoeae. We characterize the biochemical propert...

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Main Authors:	Bhoopalan, Senthil V., Piekarowicz, Andrzej, Lenz, Jonathan D., Dillard, Joseph P., Stein, Daniel C.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772129/

id	pubmed-4772129
recordtype	oai_dc
spelling	pubmed-47721292016-03-07 nagZ Triggers Gonococcal Biofilm Disassembly Bhoopalan, Senthil V. Piekarowicz, Andrzej Lenz, Jonathan D. Dillard, Joseph P. Stein, Daniel C. Article Bacterial-bacterial interactions play a critical role in promoting biofilm formation. Here we show that NagZ, a protein associated with peptidoglycan recycling, has moonlighting activity that allows it to modulate biofilm accumulation by Neisseria gonorrhoeae. We characterize the biochemical properties of NagZ and demonstrate its ability to function as a dispersing agent for biofilms formed on abiotic surfaces. We extend these observations to cell culture and tissue explant models and show that in nagZ mutants, the biofilms formed in cell culture and on human tissues contain significantly more biomass than those formed by a wild-type strain. Our results demonstrate that an enzyme thought to be restricted to peptidoglycan recycling is able to disperse preformed biofilms. Nature Publishing Group 2016-03-01 /pmc/articles/PMC4772129/ /pubmed/26927542 http://dx.doi.org/10.1038/srep22372 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bhoopalan, Senthil V. Piekarowicz, Andrzej Lenz, Jonathan D. Dillard, Joseph P. Stein, Daniel C.
spellingShingle	Bhoopalan, Senthil V. Piekarowicz, Andrzej Lenz, Jonathan D. Dillard, Joseph P. Stein, Daniel C. nagZ Triggers Gonococcal Biofilm Disassembly
author_facet	Bhoopalan, Senthil V. Piekarowicz, Andrzej Lenz, Jonathan D. Dillard, Joseph P. Stein, Daniel C.
author_sort	Bhoopalan, Senthil V.
title	nagZ Triggers Gonococcal Biofilm Disassembly
title_short	nagZ Triggers Gonococcal Biofilm Disassembly
title_full	nagZ Triggers Gonococcal Biofilm Disassembly
title_fullStr	nagZ Triggers Gonococcal Biofilm Disassembly
title_full_unstemmed	nagZ Triggers Gonococcal Biofilm Disassembly
title_sort	nagz triggers gonococcal biofilm disassembly
description	Bacterial-bacterial interactions play a critical role in promoting biofilm formation. Here we show that NagZ, a protein associated with peptidoglycan recycling, has moonlighting activity that allows it to modulate biofilm accumulation by Neisseria gonorrhoeae. We characterize the biochemical properties of NagZ and demonstrate its ability to function as a dispersing agent for biofilms formed on abiotic surfaces. We extend these observations to cell culture and tissue explant models and show that in nagZ mutants, the biofilms formed in cell culture and on human tissues contain significantly more biomass than those formed by a wild-type strain. Our results demonstrate that an enzyme thought to be restricted to peptidoglycan recycling is able to disperse preformed biofilms.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772129/
_version_	1613545113856770048

nagZ Triggers Gonococcal Biofilm Disassembly

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