Activated mast cells promote differentiation of B cells into effector cells

Based on the known accumulation of mast cells (MCs) in B cell-dependent inflammatory diseases, including rheumatoid arthritis, we hypothesized that MCs directly modulate B cells. We show here that degranulated, and to a lesser extent naïve or IgE-sensitized, MCs activate both naïve and B cell recept...

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Main Authors:	Palm, Anna-Karin E., Garcia-Faroldi, Gianni, Lundberg, Marcus, Pejler, Gunnar, Kleinau, Sandra
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742803/

id	pubmed-4742803
recordtype	oai_dc
spelling	pubmed-47428032016-02-09 Activated mast cells promote differentiation of B cells into effector cells Palm, Anna-Karin E. Garcia-Faroldi, Gianni Lundberg, Marcus Pejler, Gunnar Kleinau, Sandra Article Based on the known accumulation of mast cells (MCs) in B cell-dependent inflammatory diseases, including rheumatoid arthritis, we hypothesized that MCs directly modulate B cells. We show here that degranulated, and to a lesser extent naïve or IgE-sensitized, MCs activate both naïve and B cell receptor-activated B cells. This was shown by increased proliferation, blast formation, and expression of CD19, MHC class II and CD86 in the B cells. Further, MCs stimulated the secretion of IgM and IgG in IgM+ B cells, indicating that MCs can induce class-switch recombination in B cells. We also show that coculture of MCs with B cells promotes surface expression of L-selectin, a homing receptor, on the B cells. The effects of MCs on B cells were partly dependent on cell-cell contact and both follicular and marginal zone B cells could be activated by MCs. Our findings suggest that degranulated MCs support optimal activation of B cells, a finding that is in line with in vivo studies showing that MCs frequently degranulate in the context of B-cell driven pathologies such as arthritis. Together, our findings show that MCs have the capacity to differentiate B cells to effector cells. Nature Publishing Group 2016-02-05 /pmc/articles/PMC4742803/ /pubmed/26847186 http://dx.doi.org/10.1038/srep20531 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Palm, Anna-Karin E. Garcia-Faroldi, Gianni Lundberg, Marcus Pejler, Gunnar Kleinau, Sandra
spellingShingle	Palm, Anna-Karin E. Garcia-Faroldi, Gianni Lundberg, Marcus Pejler, Gunnar Kleinau, Sandra Activated mast cells promote differentiation of B cells into effector cells
author_facet	Palm, Anna-Karin E. Garcia-Faroldi, Gianni Lundberg, Marcus Pejler, Gunnar Kleinau, Sandra
author_sort	Palm, Anna-Karin E.
title	Activated mast cells promote differentiation of B cells into effector cells
title_short	Activated mast cells promote differentiation of B cells into effector cells
title_full	Activated mast cells promote differentiation of B cells into effector cells
title_fullStr	Activated mast cells promote differentiation of B cells into effector cells
title_full_unstemmed	Activated mast cells promote differentiation of B cells into effector cells
title_sort	activated mast cells promote differentiation of b cells into effector cells
description	Based on the known accumulation of mast cells (MCs) in B cell-dependent inflammatory diseases, including rheumatoid arthritis, we hypothesized that MCs directly modulate B cells. We show here that degranulated, and to a lesser extent naïve or IgE-sensitized, MCs activate both naïve and B cell receptor-activated B cells. This was shown by increased proliferation, blast formation, and expression of CD19, MHC class II and CD86 in the B cells. Further, MCs stimulated the secretion of IgM and IgG in IgM+ B cells, indicating that MCs can induce class-switch recombination in B cells. We also show that coculture of MCs with B cells promotes surface expression of L-selectin, a homing receptor, on the B cells. The effects of MCs on B cells were partly dependent on cell-cell contact and both follicular and marginal zone B cells could be activated by MCs. Our findings suggest that degranulated MCs support optimal activation of B cells, a finding that is in line with in vivo studies showing that MCs frequently degranulate in the context of B-cell driven pathologies such as arthritis. Together, our findings show that MCs have the capacity to differentiate B cells to effector cells.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742803/
_version_	1613534390035415040

Activated mast cells promote differentiation of B cells into effector cells

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