Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the mat...

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Main Authors:	Larrouy-Maumus, Gerald, Clements, Abigail, Filloux, Alain, McCarthy, Ronan R., Mostowy, Serge
Format:	Online
Language:	English
Published:	Elsevier Biomedical 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/

id	pubmed-4717120
recordtype	oai_dc
spelling	pubmed-47171202016-02-12 Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry Larrouy-Maumus, Gerald Clements, Abigail Filloux, Alain McCarthy, Ronan R. Mostowy, Serge Article The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry. Elsevier Biomedical 2016-01 /pmc/articles/PMC4717120/ /pubmed/26656001 http://dx.doi.org/10.1016/j.mimet.2015.12.004 Text en © 2015 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Larrouy-Maumus, Gerald Clements, Abigail Filloux, Alain McCarthy, Ronan R. Mostowy, Serge
spellingShingle	Larrouy-Maumus, Gerald Clements, Abigail Filloux, Alain McCarthy, Ronan R. Mostowy, Serge Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
author_facet	Larrouy-Maumus, Gerald Clements, Abigail Filloux, Alain McCarthy, Ronan R. Mostowy, Serge
author_sort	Larrouy-Maumus, Gerald
title	Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_short	Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_full	Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_fullStr	Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_full_unstemmed	Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_sort	direct detection of lipid a on intact gram-negative bacteria by maldi-tof mass spectrometry
description	The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
publisher	Elsevier Biomedical
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/
_version_	1613525233779605504

Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

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