Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the mat...
| Main Authors: | , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Elsevier Biomedical
2016
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/ |