Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction
Mycobacterium africanum consists of two lineages, lineages 5 and 6, within the Mycobacterium tuberculosis complex (MTC) that cause human tuberculosis in West Africa, but are found rarely outside of this region. Our analysis of the whole genome sequences of 26 lineage 5 and 6 isolates, and 66 isolate...
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Public Library of Science
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713829/ |
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pubmed-47138292016-01-26 Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction Winglee, Kathryn Manson McGuire, Abigail Maiga, Mamoudou Abeel, Thomas Shea, Terrance Desjardins, Christopher A. Diarra, Bassirou Baya, Bocar Sanogo, Moumine Diallo, Souleymane Earl, Ashlee M. Bishai, William R. Research Article Mycobacterium africanum consists of two lineages, lineages 5 and 6, within the Mycobacterium tuberculosis complex (MTC) that cause human tuberculosis in West Africa, but are found rarely outside of this region. Our analysis of the whole genome sequences of 26 lineage 5 and 6 isolates, and 66 isolates from other lineages within the MTC, reveal that M. africanum does not meet modern criteria to be considered an independent species. We analyzed drug resistance-associated genes and found that drug resistance evolves within these lineages through similar mechanisms as observed for the rest of the MTC in Mali. Though we did not see an elevated number of mutations in virulence-associated genes in these two lineages, we identified a number of lineage-specific mutations, pseudogenes and changes in gene content that may impact virulence and host specificity, and improve, overall, our understanding of what make these lineages unique. Public Library of Science 2016-01-11 /pmc/articles/PMC4713829/ /pubmed/26751217 http://dx.doi.org/10.1371/journal.pntd.0004332 Text en © 2016 Winglee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
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NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Winglee, Kathryn Manson McGuire, Abigail Maiga, Mamoudou Abeel, Thomas Shea, Terrance Desjardins, Christopher A. Diarra, Bassirou Baya, Bocar Sanogo, Moumine Diallo, Souleymane Earl, Ashlee M. Bishai, William R. |
| spellingShingle |
Winglee, Kathryn Manson McGuire, Abigail Maiga, Mamoudou Abeel, Thomas Shea, Terrance Desjardins, Christopher A. Diarra, Bassirou Baya, Bocar Sanogo, Moumine Diallo, Souleymane Earl, Ashlee M. Bishai, William R. Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| author_facet |
Winglee, Kathryn Manson McGuire, Abigail Maiga, Mamoudou Abeel, Thomas Shea, Terrance Desjardins, Christopher A. Diarra, Bassirou Baya, Bocar Sanogo, Moumine Diallo, Souleymane Earl, Ashlee M. Bishai, William R. |
| author_sort |
Winglee, Kathryn |
| title |
Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| title_short |
Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| title_full |
Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| title_fullStr |
Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| title_full_unstemmed |
Whole Genome Sequencing of Mycobacterium africanum Strains from Mali Provides Insights into the Mechanisms of Geographic Restriction |
| title_sort |
whole genome sequencing of mycobacterium africanum strains from mali provides insights into the mechanisms of geographic restriction |
| description |
Mycobacterium africanum consists of two lineages, lineages 5 and 6, within the Mycobacterium tuberculosis complex (MTC) that cause human tuberculosis in West Africa, but are found rarely outside of this region. Our analysis of the whole genome sequences of 26 lineage 5 and 6 isolates, and 66 isolates from other lineages within the MTC, reveal that M. africanum does not meet modern criteria to be considered an independent species. We analyzed drug resistance-associated genes and found that drug resistance evolves within these lineages through similar mechanisms as observed for the rest of the MTC in Mali. Though we did not see an elevated number of mutations in virulence-associated genes in these two lineages, we identified a number of lineage-specific mutations, pseudogenes and changes in gene content that may impact virulence and host specificity, and improve, overall, our understanding of what make these lineages unique. |
| publisher |
Public Library of Science |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713829/ |
| _version_ |
1613524254900355072 |