The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering

The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering

Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites....

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Main Authors: Çamdere, Gamze, Guacci, Vincent, Stricklin, Jeremiah, Koshland, Douglas
Format: Online
Language:English
Published: eLife Sciences Publications, Ltd 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709263/
id pubmed-4709263
recordtype oai_dc
spelling pubmed-47092632016-01-13 The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering Çamdere, Gamze Guacci, Vincent Stricklin, Jeremiah Koshland, Douglas Biochemistry Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites. These ATPases are required for cohesin to bind DNA. Cohesin’s DNA binding activity is also promoted by the Eco1 acetyltransferase and inhibited by Wpl1. Recently we showed that after cohesin stably binds DNA, a second step is required for DNA tethering. This second step is also controlled by Eco1 acetylation. Here, we use genetic and biochemical analyses to show that this second DNA tethering step is regulated by cohesin ATPase. Furthermore, our results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition. eLife Sciences Publications, Ltd 2015-11-19 /pmc/articles/PMC4709263/ /pubmed/26583750 http://dx.doi.org/10.7554/eLife.11315 Text en © 2015, Çamdere et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Çamdere, Gamze
Guacci, Vincent
Stricklin, Jeremiah
Koshland, Douglas
spellingShingle Çamdere, Gamze
Guacci, Vincent
Stricklin, Jeremiah
Koshland, Douglas
The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
author_facet Çamdere, Gamze
Guacci, Vincent
Stricklin, Jeremiah
Koshland, Douglas
author_sort Çamdere, Gamze
title The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
title_short The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
title_full The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
title_fullStr The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
title_full_unstemmed The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering
title_sort atpases of cohesin interface with regulators to modulate cohesin-mediated dna tethering
description Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites. These ATPases are required for cohesin to bind DNA. Cohesin’s DNA binding activity is also promoted by the Eco1 acetyltransferase and inhibited by Wpl1. Recently we showed that after cohesin stably binds DNA, a second step is required for DNA tethering. This second step is also controlled by Eco1 acetylation. Here, we use genetic and biochemical analyses to show that this second DNA tethering step is regulated by cohesin ATPase. Furthermore, our results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition.
publisher eLife Sciences Publications, Ltd
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709263/
_version_ 1613522805764128768

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