Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia

The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, a...

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Main Authors:	Tokuda, Shinsaku, Kim, Young Hak, Matsumoto, Hisako, Muro, Shigeo, Hirai, Toyohiro, Mishima, Michiaki, Furuse, Mikio
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696811/

id	pubmed-4696811
recordtype	oai_dc
spelling	pubmed-46968112016-01-13 Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia Tokuda, Shinsaku Kim, Young Hak Matsumoto, Hisako Muro, Shigeo Hirai, Toyohiro Mishima, Michiaki Furuse, Mikio Research Article The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, and the relationship between elevated hydrostatic pressure and cell properties related to malignant tumors is less well understood. Therefore, we investigated the effects of hydrostatic pressure on the cultured epithelial cells seeded on permeable filters. Surprisingly, hydrostatic pressure from basal to apical side induced epithelial stratification in Madin-Darby canine kidney (MDCK) I and Caco-2 cells, and cavities with microvilli and tight junctions around their surfaces were formed within the multi-layered epithelia. The hydrostatic pressure gradient also promoted cell proliferation, suppressed cell apoptosis, and increased transepithelial ion permeability. The inhibition of protein kinase A (PKA) promoted epithelial stratification by the hydrostatic pressure whereas the activation of PKA led to suppressed epithelial stratification. These results indicate the role of the hydrostatic pressure gradient in the regulation of various epithelial cell functions. The findings in this study may provide clues for the development of a novel strategy for the treatment of the carcinoma. Public Library of Science 2015-12-30 /pmc/articles/PMC4696811/ /pubmed/26716691 http://dx.doi.org/10.1371/journal.pone.0145522 Text en © 2015 Tokuda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tokuda, Shinsaku Kim, Young Hak Matsumoto, Hisako Muro, Shigeo Hirai, Toyohiro Mishima, Michiaki Furuse, Mikio
spellingShingle	Tokuda, Shinsaku Kim, Young Hak Matsumoto, Hisako Muro, Shigeo Hirai, Toyohiro Mishima, Michiaki Furuse, Mikio Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
author_facet	Tokuda, Shinsaku Kim, Young Hak Matsumoto, Hisako Muro, Shigeo Hirai, Toyohiro Mishima, Michiaki Furuse, Mikio
author_sort	Tokuda, Shinsaku
title	Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
title_short	Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
title_full	Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
title_fullStr	Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
title_full_unstemmed	Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia
title_sort	effects of hydrostatic pressure on carcinogenic properties of epithelia
description	The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, and the relationship between elevated hydrostatic pressure and cell properties related to malignant tumors is less well understood. Therefore, we investigated the effects of hydrostatic pressure on the cultured epithelial cells seeded on permeable filters. Surprisingly, hydrostatic pressure from basal to apical side induced epithelial stratification in Madin-Darby canine kidney (MDCK) I and Caco-2 cells, and cavities with microvilli and tight junctions around their surfaces were formed within the multi-layered epithelia. The hydrostatic pressure gradient also promoted cell proliferation, suppressed cell apoptosis, and increased transepithelial ion permeability. The inhibition of protein kinase A (PKA) promoted epithelial stratification by the hydrostatic pressure whereas the activation of PKA led to suppressed epithelial stratification. These results indicate the role of the hydrostatic pressure gradient in the regulation of various epithelial cell functions. The findings in this study may provide clues for the development of a novel strategy for the treatment of the carcinoma.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696811/
_version_	1613518517329461248

Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia

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