Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy
Introduction: Current gene therapy involves replacement of defective gene by delivery of healthy genetic material to precede normal function. Virus-mediated gene delivery is the most successful and efficient method for gene therapy, but it has been challenged due to serious safety concerns. Convers...
| Main Authors: | , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Informa Healthcare
2015
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696419/ |
| id |
pubmed-4696419 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-46964192016-01-12 Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy Youn, Hyewon Chung, June-Key Review Introduction: Current gene therapy involves replacement of defective gene by delivery of healthy genetic material to precede normal function. Virus-mediated gene delivery is the most successful and efficient method for gene therapy, but it has been challenged due to serious safety concerns. Conversely, gene delivery using plasmid DNA (pDNA) is considered safer, but its transfection efficiency is much lower than virus-mediated gene transfer. Recently, mRNA has been suggested as an alternative option to avoid undesired insertion of delivered DNA sequences with higher transfection efficiency and stability. Informa Healthcare 2015-09-02 2015-06-30 /pmc/articles/PMC4696419/ /pubmed/26125492 http://dx.doi.org/10.1517/14712598.2015.1057563 Text en © The Author(s). Published by Informa UK, Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Youn, Hyewon Chung, June-Key |
| spellingShingle |
Youn, Hyewon Chung, June-Key Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| author_facet |
Youn, Hyewon Chung, June-Key |
| author_sort |
Youn, Hyewon |
| title |
Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| title_short |
Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| title_full |
Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| title_fullStr |
Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| title_full_unstemmed |
Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy |
| title_sort |
modified mrna as an alternative to plasmid dna (pdna) for transcript replacement and vaccination therapy |
| description |
Introduction: Current gene therapy involves replacement of defective gene by delivery of healthy genetic material to precede normal function. Virus-mediated gene delivery is the most successful and efficient method for gene therapy, but it has been challenged due to serious safety concerns. Conversely, gene delivery using plasmid DNA (pDNA) is considered safer, but its transfection efficiency is much lower than virus-mediated gene transfer. Recently, mRNA has been suggested as an alternative option to avoid undesired insertion of delivered DNA sequences with higher transfection efficiency and stability. |
| publisher |
Informa Healthcare |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696419/ |
| _version_ |
1613518411340447744 |