Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations

The data in this article are related to the research article entitled “Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations” Botezatu et al. [1]. Somatic mutations in the PIK3CA gene (“hot spots” in exons 9 and 20) are found in many human cancers, and t...

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Main Authors:	Botezatu, Irina V., Nechaeva, Irina O., Stroganova, Аnna М., Senderovich, Anastasia I., Kondratova, Valentina N., Shelepov, Valery P., Lichtenstein, Anatoly V.
Format:	Online
Language:	English
Published:	Elsevier 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669479/

id	pubmed-4669479
recordtype	oai_dc
spelling	pubmed-46694792015-12-23 Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations Botezatu, Irina V. Nechaeva, Irina O. Stroganova, Аnna М. Senderovich, Anastasia I. Kondratova, Valentina N. Shelepov, Valery P. Lichtenstein, Anatoly V. Data Article The data in this article are related to the research article entitled “Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations” Botezatu et al. [1]. Somatic mutations in the PIK3CA gene (“hot spots” in exons 9 and 20) are found in many human cancers, and their presence can determine prognosis and a treatment strategy. An effective method of mutation scanning PIK3CA in clinical laboratories is DNA Melting Analysis (DMA) (Vorkas et al., 2010; Simi et al., 2008) [2], [3]. It was demonstrated recently that the TaqMan probes which have been long used in Real Time PCR may also be utilized in DMA (Huang et al., 2011) [4]. After optimization of this method Botezatu et al. [1], it was used for multiplex scanning PIK3CA hotspot mutations in formalin-fixed paraffin-embedded (FFPE) samples from patients with colorectal and lung cancer. Elsevier 2015-11-14 /pmc/articles/PMC4669479/ /pubmed/26702420 http://dx.doi.org/10.1016/j.dib.2015.10.046 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Botezatu, Irina V. Nechaeva, Irina O. Stroganova, Аnna М. Senderovich, Anastasia I. Kondratova, Valentina N. Shelepov, Valery P. Lichtenstein, Anatoly V.
spellingShingle	Botezatu, Irina V. Nechaeva, Irina O. Stroganova, Аnna М. Senderovich, Anastasia I. Kondratova, Valentina N. Shelepov, Valery P. Lichtenstein, Anatoly V. Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
author_facet	Botezatu, Irina V. Nechaeva, Irina O. Stroganova, Аnna М. Senderovich, Anastasia I. Kondratova, Valentina N. Shelepov, Valery P. Lichtenstein, Anatoly V.
author_sort	Botezatu, Irina V.
title	Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
title_short	Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
title_full	Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
title_fullStr	Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
title_full_unstemmed	Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
title_sort	asymmetric real-time pcr and multiplex melting curve analysis with taqman probes for detecting pik3ca mutations
description	The data in this article are related to the research article entitled “Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations” Botezatu et al. [1]. Somatic mutations in the PIK3CA gene (“hot spots” in exons 9 and 20) are found in many human cancers, and their presence can determine prognosis and a treatment strategy. An effective method of mutation scanning PIK3CA in clinical laboratories is DNA Melting Analysis (DMA) (Vorkas et al., 2010; Simi et al., 2008) [2], [3]. It was demonstrated recently that the TaqMan probes which have been long used in Real Time PCR may also be utilized in DMA (Huang et al., 2011) [4]. After optimization of this method Botezatu et al. [1], it was used for multiplex scanning PIK3CA hotspot mutations in formalin-fixed paraffin-embedded (FFPE) samples from patients with colorectal and lung cancer.
publisher	Elsevier
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669479/
_version_	1613509460708294656

Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations

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