Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chrom...
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Associação Brasileira de Divulgação Científica
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617116/ |
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pubmed-46171162015-11-24 Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+ Faria, D.P. Buchpiguel, C.A. Marques, F.L.N. IX Meeting of the "Sociedade Brasileira De Biociências Nucleares". Caxambu, Mg, Brazil August 27-30, 2014 Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol:chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI)6]+ since impurities such as99mTc-reduced-hydrolyzed (RH),99mTcO4 - and [99mTc(cysteine)2]-complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines. Associação Brasileira de Divulgação Científica 2015-03-03 /pmc/articles/PMC4617116/ /pubmed/26445333 http://dx.doi.org/10.1590/1414-431X20144489 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Faria, D.P. Buchpiguel, C.A. Marques, F.L.N. |
| spellingShingle |
Faria, D.P. Buchpiguel, C.A. Marques, F.L.N. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+ |
| author_facet |
Faria, D.P. Buchpiguel, C.A. Marques, F.L.N. |
| author_sort |
Faria, D.P. |
| title |
Alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mTc(MIBI)6]+
|
| title_short |
Alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mTc(MIBI)6]+
|
| title_full |
Alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mTc(MIBI)6]+
|
| title_fullStr |
Alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mTc(MIBI)6]+
|
| title_full_unstemmed |
Alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mTc(MIBI)6]+
|
| title_sort |
alternative chromatographic system for the quality control of lipophilic
technetium-99m radiopharmaceuticals such as
[99mtc(mibi)6]+ |
| description |
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be
evaluated by several methods and techniques. Planar chromatography is the technique
normally employed in nuclear medicine since it is simple, rapid and usually of low
cost. There is no standard system for the chromatographic technique, but price,
separation efficiency and short time for execution must be considered. We have
studied an alternative system using common chromatographic stationary phase and
alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic
radiopharmaceutical [99mTc(MIBI)6]+ as a model.
Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and
methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and
the more expensive ITLC-SG and 1-propanol:chloroform (10:90) were suitable systems
for the direct determination of radiochemical purity of
[99mTc(MIBI)6]+ since impurities such
as99mTc-reduced-hydrolyzed (RH),99mTcO4
- and [99mTc(cysteine)2]-complex were
completely separated from the radiopharmaceutical, which moved toward the front of
chromatographic systems while impurities were retained at the origin. The time
required for analysis was 4 to 15 min, which is appropriate for nuclear medicine
routines. |
| publisher |
Associação Brasileira de Divulgação Científica |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617116/ |
| _version_ |
1613491782221299712 |