Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping

We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mo...

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Main Authors:	Yoon, Jonghee, Lee, Minji, Lee, KyeoReh, Kim, Nury, Kim, Jin Man, Park, Jongchan, Yu, Hyeonseung, Choi, Chulhee, Heo, Won Do, Park, YongKeun
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543936/

id	pubmed-4543936
recordtype	oai_dc
spelling	pubmed-45439362015-09-01 Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping Yoon, Jonghee Lee, Minji Lee, KyeoReh Kim, Nury Kim, Jin Man Park, Jongchan Yu, Hyeonseung Choi, Chulhee Heo, Won Do Park, YongKeun Article We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca2+ level at the individual-cell level. Nature Publishing Group 2015-08-21 /pmc/articles/PMC4543936/ /pubmed/26293590 http://dx.doi.org/10.1038/srep13289 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yoon, Jonghee Lee, Minji Lee, KyeoReh Kim, Nury Kim, Jin Man Park, Jongchan Yu, Hyeonseung Choi, Chulhee Heo, Won Do Park, YongKeun
spellingShingle	Yoon, Jonghee Lee, Minji Lee, KyeoReh Kim, Nury Kim, Jin Man Park, Jongchan Yu, Hyeonseung Choi, Chulhee Heo, Won Do Park, YongKeun Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
author_facet	Yoon, Jonghee Lee, Minji Lee, KyeoReh Kim, Nury Kim, Jin Man Park, Jongchan Yu, Hyeonseung Choi, Chulhee Heo, Won Do Park, YongKeun
author_sort	Yoon, Jonghee
title	Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_short	Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_full	Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_fullStr	Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_full_unstemmed	Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
title_sort	optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
description	We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and to focus light on the target cells. We demonstrate that illumination with shaped waves enables spatiotemporal regulation of intracellular Ca2+ level at the individual-cell level.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543936/
_version_	1613261931562401792

Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping

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