In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry

In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry

The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of...

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Main Authors: Torper, Olof, Ottosson, Daniella Rylander, Pereira, Maria, Lau, Shong, Cardoso, Tiago, Grealish, Shane, Parmar, Malin
Format: Online
Language:English
Published: Cell Press 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521079/
id pubmed-4521079
recordtype oai_dc
spelling pubmed-45210792015-08-01 In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry Torper, Olof Ottosson, Daniella Rylander Pereira, Maria Lau, Shong Cardoso, Tiago Grealish, Shane Parmar, Malin Article The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain. Cell Press 2015-07-09 /pmc/articles/PMC4521079/ /pubmed/26166567 http://dx.doi.org/10.1016/j.celrep.2015.06.040 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Torper, Olof
Ottosson, Daniella Rylander
Pereira, Maria
Lau, Shong
Cardoso, Tiago
Grealish, Shane
Parmar, Malin
spellingShingle Torper, Olof
Ottosson, Daniella Rylander
Pereira, Maria
Lau, Shong
Cardoso, Tiago
Grealish, Shane
Parmar, Malin
In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
author_facet Torper, Olof
Ottosson, Daniella Rylander
Pereira, Maria
Lau, Shong
Cardoso, Tiago
Grealish, Shane
Parmar, Malin
author_sort Torper, Olof
title In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
title_short In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
title_full In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
title_fullStr In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
title_full_unstemmed In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry
title_sort in vivo reprogramming of striatal ng2 glia into functional neurons that integrate into local host circuitry
description The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain.
publisher Cell Press
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521079/
_version_ 1613254465065844736

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