Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells

The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes achieved by lentiviral transduction. To date, ef...

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Bibliographic Details
Main Authors: Witkowski, Wojciech, Vermeire, Jolien, Landi, Alessia, Naessens, Evelien, Vanderstraeten, Hanne, Nauwynck, Hans, Favoreel, Herman, Verhasselt, Bruno
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514788/