Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dyn...

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Bibliographic Details
Main Authors: Vermeire, Jolien, Naessens, Evelien, Vanderstraeten, Hanne, Landi, Alessia, Iannucci, Veronica, Van Nuffel, Anouk, Taghon, Tom, Pizzato, Massimo, Verhasselt, Bruno
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515444/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515444/

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