The Hill analysis and co-ion–driven transporter kinetics

Interaction of multiple ligands with a protein or protein complex is a widespread phenomenon that allows for cooperativity. Here, we review the use of the Hill equation, which is commonly used to analyze binding or kinetic data, to analyze the kinetics of ion-coupled transporters and show how the me...

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Main Authors:	Lolkema, Juke S., Slotboom, Dirk-Jan
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442790/

id	pubmed-4442790
recordtype	oai_dc
spelling	pubmed-44427902015-12-01 The Hill analysis and co-ion–driven transporter kinetics Lolkema, Juke S. Slotboom, Dirk-Jan Tutorial Interaction of multiple ligands with a protein or protein complex is a widespread phenomenon that allows for cooperativity. Here, we review the use of the Hill equation, which is commonly used to analyze binding or kinetic data, to analyze the kinetics of ion-coupled transporters and show how the mechanism of transport affects the Hill coefficient. Importantly, the Hill analysis of ion-coupled transporters can provide the exact number of transported co-ions, regardless of the extent of the cooperativity in ion binding. The Rockefeller University Press 2015-06 /pmc/articles/PMC4442790/ /pubmed/26009547 http://dx.doi.org/10.1085/jgp.201411332 Text en © 2015 Lolkema and Slotboom This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lolkema, Juke S. Slotboom, Dirk-Jan
spellingShingle	Lolkema, Juke S. Slotboom, Dirk-Jan The Hill analysis and co-ion–driven transporter kinetics
author_facet	Lolkema, Juke S. Slotboom, Dirk-Jan
author_sort	Lolkema, Juke S.
title	The Hill analysis and co-ion–driven transporter kinetics
title_short	The Hill analysis and co-ion–driven transporter kinetics
title_full	The Hill analysis and co-ion–driven transporter kinetics
title_fullStr	The Hill analysis and co-ion–driven transporter kinetics
title_full_unstemmed	The Hill analysis and co-ion–driven transporter kinetics
title_sort	hill analysis and co-ion–driven transporter kinetics
description	Interaction of multiple ligands with a protein or protein complex is a widespread phenomenon that allows for cooperativity. Here, we review the use of the Hill equation, which is commonly used to analyze binding or kinetic data, to analyze the kinetics of ion-coupled transporters and show how the mechanism of transport affects the Hill coefficient. Importantly, the Hill analysis of ion-coupled transporters can provide the exact number of transported co-ions, regardless of the extent of the cooperativity in ion binding.
publisher	The Rockefeller University Press
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442790/
_version_	1613227306713612288

The Hill analysis and co-ion–driven transporter kinetics

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