Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells

Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells

DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases in human embryonic stem cells (ESCs) using CRISPR/Cas9 genome editing to further investigate their role...

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Bibliographic Details
Main Authors: Liao, Jing, Karnik, Rahul, Gu, Hongcang, Ziller, Michael J., Clement, Kendell, Tsankov, Alexander M., Akopian, Veronika, Gifford, Casey A., Donaghey, Julie, Galonska, Christina, Pop, Ramona, Reyon, Deepak, Tsai, Shengdar Q., Mallard, William, Joung, J. Keith, Rinn, John L., Gnirke, Andreas, Meissner, Alexander
Format: Online
Language:English
Published: 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414868/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414868/

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