TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition
The cell adhesion glycoproteins, TgrB1 and TgrC1, are essential for Dictyostelium development and allorecognition, but it has been impossible to determine whether their pleiotropic roles are due to one common function or to distinct functions in separate pathways. Mutations in the respective genes,...
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| Format: | Online |
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Public Library of Science
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404348/ |
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pubmed-44043482015-05-02 TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition Wang, Yue Shaulsky, Gad Research Article The cell adhesion glycoproteins, TgrB1 and TgrC1, are essential for Dictyostelium development and allorecognition, but it has been impossible to determine whether their pleiotropic roles are due to one common function or to distinct functions in separate pathways. Mutations in the respective genes, tgrB1 and tgrC1, abrogate both development and allorecognition and the defects cannot be suppressed by activation of the cyclic AMP dependent protein kinase PKA, a central regulator of Dictyostelium development. Here we report that mutations in genes outside the known PKA pathway partially suppress the tgrC1-null developmental defect. We separated the pleiotropic roles of tgrC1 by testing the effects of a suppression mutation, stcinsA under different conditions. stcAins modified only the developmental defect of tgrC1– but not the allorecognition defect, suggesting that the two functions are separable. The suppressor mutant phenotype also revealed that tgrC1 regulates stalk differentiation in a cell-autonomous manner and spore differentiation in a non-cell-autonomous manner. Moreover, stcAins did not modify the developmental defect of tgrB1–, but the less robust phenotype of tgrB1– obscures the possible role of stcA relative to tgrB1. Public Library of Science 2015-04-20 /pmc/articles/PMC4404348/ /pubmed/25894230 http://dx.doi.org/10.1371/journal.pone.0124270 Text en © 2015 Wang, Shaulsky http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wang, Yue Shaulsky, Gad |
| spellingShingle |
Wang, Yue Shaulsky, Gad TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| author_facet |
Wang, Yue Shaulsky, Gad |
| author_sort |
Wang, Yue |
| title |
TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| title_short |
TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| title_full |
TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| title_fullStr |
TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| title_full_unstemmed |
TgrC1 Has Distinct Functions in Dictyostelium Development and Allorecognition |
| title_sort |
tgrc1 has distinct functions in dictyostelium development and allorecognition |
| description |
The cell adhesion glycoproteins, TgrB1 and TgrC1, are essential for Dictyostelium development and allorecognition, but it has been impossible to determine whether their pleiotropic roles are due to one common function or to distinct functions in separate pathways. Mutations in the respective genes, tgrB1 and tgrC1, abrogate both development and allorecognition and the defects cannot be suppressed by activation of the cyclic AMP dependent protein kinase PKA, a central regulator of Dictyostelium development. Here we report that mutations in genes outside the known PKA pathway partially suppress the tgrC1-null developmental defect. We separated the pleiotropic roles of tgrC1 by testing the effects of a suppression mutation, stcinsA under different conditions. stcAins modified only the developmental defect of tgrC1– but not the allorecognition defect, suggesting that the two functions are separable. The suppressor mutant phenotype also revealed that tgrC1 regulates stalk differentiation in a cell-autonomous manner and spore differentiation in a non-cell-autonomous manner. Moreover, stcAins did not modify the developmental defect of tgrB1–, but the less robust phenotype of tgrB1– obscures the possible role of stcA relative to tgrB1. |
| publisher |
Public Library of Science |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404348/ |
| _version_ |
1613213766459064320 |