Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos
•Targeted genomic deletions can be generated directly in one-cell mouse embryos using sequence-specific nucleases.•Deletions occur by end ligation repair and are not supported by homologous recombination.•TALEN as well as CRISPR/Cas nucleases can be used.•Homozygous mutants exhibiting phenotypes can...
| Main Authors: | , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Elsevier
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309836/ |