Telomerase Reverse Transcriptase Regulates microRNAs

MicroRNAs are small non-coding RNAs that inhibit the translation of target mRNAs. In humans, most microRNAs are transcribed by RNA polymerase II as long primary transcripts and processed by sequential cleavage of the two RNase III enzymes, DROSHA and DICER, into precursor and mature microRNAs, respe...

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Main Authors:	Lassmann, Timo, Maida, Yoshiko, Tomaru, Yasuhiro, Yasukawa, Mami, Ando, Yoshinari, Kojima, Miki, Kasim, Vivi, Simon, Christophe, Daub, Carsten O., Carninci, Piero, Hayashizaki, Yoshihide, Masutomi, Kenkichi
Format:	Online
Language:	English
Published:	MDPI 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307298/

id	pubmed-4307298
recordtype	oai_dc
spelling	pubmed-43072982015-02-02 Telomerase Reverse Transcriptase Regulates microRNAs Lassmann, Timo Maida, Yoshiko Tomaru, Yasuhiro Yasukawa, Mami Ando, Yoshinari Kojima, Miki Kasim, Vivi Simon, Christophe Daub, Carsten O. Carninci, Piero Hayashizaki, Yoshihide Masutomi, Kenkichi Article MicroRNAs are small non-coding RNAs that inhibit the translation of target mRNAs. In humans, most microRNAs are transcribed by RNA polymerase II as long primary transcripts and processed by sequential cleavage of the two RNase III enzymes, DROSHA and DICER, into precursor and mature microRNAs, respectively. Although the fundamental functions of microRNAs in RNA silencing have been gradually uncovered, less is known about the regulatory mechanisms of microRNA expression. Here, we report that telomerase reverse transcriptase (TERT) extensively affects the expression levels of mature microRNAs. Deep sequencing-based screens of short RNA populations revealed that the suppression of TERT resulted in the downregulation of microRNAs expressed in THP-1 cells and HeLa cells. Primary and precursor microRNA levels were also reduced under the suppression of TERT. Similar results were obtained with the suppression of either BRG1 (also called SMARCA4) or nucleostemin, which are proteins interacting with TERT and functioning beyond telomeres. These results suggest that TERT regulates microRNAs at the very early phases in their biogenesis, presumably through non-telomerase mechanism(s). MDPI 2015-01-06 /pmc/articles/PMC4307298/ /pubmed/25569094 http://dx.doi.org/10.3390/ijms16011192 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lassmann, Timo Maida, Yoshiko Tomaru, Yasuhiro Yasukawa, Mami Ando, Yoshinari Kojima, Miki Kasim, Vivi Simon, Christophe Daub, Carsten O. Carninci, Piero Hayashizaki, Yoshihide Masutomi, Kenkichi
spellingShingle	Lassmann, Timo Maida, Yoshiko Tomaru, Yasuhiro Yasukawa, Mami Ando, Yoshinari Kojima, Miki Kasim, Vivi Simon, Christophe Daub, Carsten O. Carninci, Piero Hayashizaki, Yoshihide Masutomi, Kenkichi Telomerase Reverse Transcriptase Regulates microRNAs
author_facet	Lassmann, Timo Maida, Yoshiko Tomaru, Yasuhiro Yasukawa, Mami Ando, Yoshinari Kojima, Miki Kasim, Vivi Simon, Christophe Daub, Carsten O. Carninci, Piero Hayashizaki, Yoshihide Masutomi, Kenkichi
author_sort	Lassmann, Timo
title	Telomerase Reverse Transcriptase Regulates microRNAs
title_short	Telomerase Reverse Transcriptase Regulates microRNAs
title_full	Telomerase Reverse Transcriptase Regulates microRNAs
title_fullStr	Telomerase Reverse Transcriptase Regulates microRNAs
title_full_unstemmed	Telomerase Reverse Transcriptase Regulates microRNAs
title_sort	telomerase reverse transcriptase regulates micrornas
description	MicroRNAs are small non-coding RNAs that inhibit the translation of target mRNAs. In humans, most microRNAs are transcribed by RNA polymerase II as long primary transcripts and processed by sequential cleavage of the two RNase III enzymes, DROSHA and DICER, into precursor and mature microRNAs, respectively. Although the fundamental functions of microRNAs in RNA silencing have been gradually uncovered, less is known about the regulatory mechanisms of microRNA expression. Here, we report that telomerase reverse transcriptase (TERT) extensively affects the expression levels of mature microRNAs. Deep sequencing-based screens of short RNA populations revealed that the suppression of TERT resulted in the downregulation of microRNAs expressed in THP-1 cells and HeLa cells. Primary and precursor microRNA levels were also reduced under the suppression of TERT. Similar results were obtained with the suppression of either BRG1 (also called SMARCA4) or nucleostemin, which are proteins interacting with TERT and functioning beyond telomeres. These results suggest that TERT regulates microRNAs at the very early phases in their biogenesis, presumably through non-telomerase mechanism(s).
publisher	MDPI
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307298/
_version_	1613181026094284800

Telomerase Reverse Transcriptase Regulates microRNAs

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