The impact of dietary isoflavonoids on malignant brain tumors

Poor prognosis and limited therapeutic options render malignant brain tumors one of the most devastating diseases in clinical medicine. Current treatment strategies attempt to expand the therapeutic repertoire through the use of multimodal treatment regimens. It is here that dietary fibers have been...

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Main Authors:	Sehm, Tina, Fan, Zheng, Weiss, Ruth, Schwarz, Marc, Engelhorn, Tobias, Hore, Nirjhar, Doerfler, Arnd, Buchfelder, Michael, Eyüpoglu, IIker Y, Savaskan, Nic E
Format:	Online
Language:	English
Published:	BlackWell Publishing Ltd 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303154/

id	pubmed-4303154
recordtype	oai_dc
spelling	pubmed-43031542015-01-22 The impact of dietary isoflavonoids on malignant brain tumors Sehm, Tina Fan, Zheng Weiss, Ruth Schwarz, Marc Engelhorn, Tobias Hore, Nirjhar Doerfler, Arnd Buchfelder, Michael Eyüpoglu, IIker Y Savaskan, Nic E Cancer Biology Poor prognosis and limited therapeutic options render malignant brain tumors one of the most devastating diseases in clinical medicine. Current treatment strategies attempt to expand the therapeutic repertoire through the use of multimodal treatment regimens. It is here that dietary fibers have been recently recognized as a supportive natural therapy in augmenting the body's response to tumor growth. Here, we investigated the impact of isoflavonoids on primary brain tumor cells. First, we treated glioma cell lines and primary astrocytes with various isoflavonoids and phytoestrogens. Cell viability in a dose-dependent manner was measured for biochanin A (BCA), genistein (GST), and secoisolariciresinol diglucoside (SDG). Dose–response action for the different isoflavonoids showed that BCA is highly effective on glioma cells and nontoxic for normal differentiated brain tissues. We further investigated BCA in ex vivo and in vivo experimentations. Organotypic brain slice cultures were performed and treated with BCA. For in vivo experiments, BCA was intraperitoneal injected in tumor-implanted Fisher rats. Tumor size and edema were measured and quantified by magnetic resonance imaging (MRI) scans. In vascular organotypic glioma brain slice cultures (VOGIM) we found that BCA operates antiangiogenic and neuroprotective. In vivo MRI scans demonstrated that administered BCA as a monotherapy was effective in reducing significantly tumor-induced brain edema and showed a trend for prolonged survival. Our results revealed that dietary isoflavonoids, in particular BCA, execute toxicity toward glioma cells, antiangiogenic, and coevally neuroprotective properties, and therefore augment the range of state-of-the-art multimodal treatment approach. BlackWell Publishing Ltd 2014-08 2014-06-04 /pmc/articles/PMC4303154/ /pubmed/24898306 http://dx.doi.org/10.1002/cam4.265 Text en © 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sehm, Tina Fan, Zheng Weiss, Ruth Schwarz, Marc Engelhorn, Tobias Hore, Nirjhar Doerfler, Arnd Buchfelder, Michael Eyüpoglu, IIker Y Savaskan, Nic E
spellingShingle	Sehm, Tina Fan, Zheng Weiss, Ruth Schwarz, Marc Engelhorn, Tobias Hore, Nirjhar Doerfler, Arnd Buchfelder, Michael Eyüpoglu, IIker Y Savaskan, Nic E The impact of dietary isoflavonoids on malignant brain tumors
author_facet	Sehm, Tina Fan, Zheng Weiss, Ruth Schwarz, Marc Engelhorn, Tobias Hore, Nirjhar Doerfler, Arnd Buchfelder, Michael Eyüpoglu, IIker Y Savaskan, Nic E
author_sort	Sehm, Tina
title	The impact of dietary isoflavonoids on malignant brain tumors
title_short	The impact of dietary isoflavonoids on malignant brain tumors
title_full	The impact of dietary isoflavonoids on malignant brain tumors
title_fullStr	The impact of dietary isoflavonoids on malignant brain tumors
title_full_unstemmed	The impact of dietary isoflavonoids on malignant brain tumors
title_sort	impact of dietary isoflavonoids on malignant brain tumors
description	Poor prognosis and limited therapeutic options render malignant brain tumors one of the most devastating diseases in clinical medicine. Current treatment strategies attempt to expand the therapeutic repertoire through the use of multimodal treatment regimens. It is here that dietary fibers have been recently recognized as a supportive natural therapy in augmenting the body's response to tumor growth. Here, we investigated the impact of isoflavonoids on primary brain tumor cells. First, we treated glioma cell lines and primary astrocytes with various isoflavonoids and phytoestrogens. Cell viability in a dose-dependent manner was measured for biochanin A (BCA), genistein (GST), and secoisolariciresinol diglucoside (SDG). Dose–response action for the different isoflavonoids showed that BCA is highly effective on glioma cells and nontoxic for normal differentiated brain tissues. We further investigated BCA in ex vivo and in vivo experimentations. Organotypic brain slice cultures were performed and treated with BCA. For in vivo experiments, BCA was intraperitoneal injected in tumor-implanted Fisher rats. Tumor size and edema were measured and quantified by magnetic resonance imaging (MRI) scans. In vascular organotypic glioma brain slice cultures (VOGIM) we found that BCA operates antiangiogenic and neuroprotective. In vivo MRI scans demonstrated that administered BCA as a monotherapy was effective in reducing significantly tumor-induced brain edema and showed a trend for prolonged survival. Our results revealed that dietary isoflavonoids, in particular BCA, execute toxicity toward glioma cells, antiangiogenic, and coevally neuroprotective properties, and therefore augment the range of state-of-the-art multimodal treatment approach.
publisher	BlackWell Publishing Ltd
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303154/
_version_	1613179649381105664

The impact of dietary isoflavonoids on malignant brain tumors

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