Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli
•Expression of recombinant human carboxylesterase I in E. coli is mainly insoluble.•Refolding using a combination of 1% glycerol and 2 mM β-mercaptoethanol in Tris–HCl, pH 7.5 significantly improved solubility.•Purified recombinant human CES1 is functionally active and stable.•We provided efficient...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294421/ |