Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons
Modern molecular genetics studies necessitate the manipulation of genes in their endogenous locus, but most of the current methodologies require an inefficient donor-dependent homologous recombination step to locally modify the genome. Here we describe a methodology to efficiently generate Drosophil...
| Main Authors: | , , , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Genetics Society of America
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267933/ |