Serotonin receptor 3A controls interneuron migration into the neocortex

Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic s...

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Main Authors:	Murthy, Sahana, Niquille, Mathieu, Hurni, Nicolas, Limoni, Greta, Frazer, Sarah, Chameau, Pascal, van Hooft, Johannes A., Vitalis, Tania, Dayer, Alexandre
Format:	Online
Language:	English
Published:	Nature Pub. Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263148/

id	pubmed-4263148
recordtype	oai_dc
spelling	pubmed-42631482014-12-16 Serotonin receptor 3A controls interneuron migration into the neocortex Murthy, Sahana Niquille, Mathieu Hurni, Nicolas Limoni, Greta Frazer, Sarah Chameau, Pascal van Hooft, Johannes A. Vitalis, Tania Dayer, Alexandre Article Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic serotonin receptor 3A (5-HT3AR) is specifically expressed in CGE-derived migrating interneurons and upregulated while they invade the developing cortex. Functional investigations using calcium imaging, electrophysiological recordings and migration assays indicate that CGE-derived INs increase their response to 5-HT3AR activation during the late phase of cortical plate invasion. Using genetic loss-of-function approaches and in vivo grafts, we further demonstrate that the 5-HT3AR is cell autonomously required for the migration and proper positioning of reelin-expressing CGE-derived INs in the neocortex. Our findings reveal a requirement for a serotonin receptor in controlling the migration and laminar positioning of a specific subtype of cortical IN. Nature Pub. Group 2014-11-20 /pmc/articles/PMC4263148/ /pubmed/25409778 http://dx.doi.org/10.1038/ncomms6524 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Murthy, Sahana Niquille, Mathieu Hurni, Nicolas Limoni, Greta Frazer, Sarah Chameau, Pascal van Hooft, Johannes A. Vitalis, Tania Dayer, Alexandre
spellingShingle	Murthy, Sahana Niquille, Mathieu Hurni, Nicolas Limoni, Greta Frazer, Sarah Chameau, Pascal van Hooft, Johannes A. Vitalis, Tania Dayer, Alexandre Serotonin receptor 3A controls interneuron migration into the neocortex
author_facet	Murthy, Sahana Niquille, Mathieu Hurni, Nicolas Limoni, Greta Frazer, Sarah Chameau, Pascal van Hooft, Johannes A. Vitalis, Tania Dayer, Alexandre
author_sort	Murthy, Sahana
title	Serotonin receptor 3A controls interneuron migration into the neocortex
title_short	Serotonin receptor 3A controls interneuron migration into the neocortex
title_full	Serotonin receptor 3A controls interneuron migration into the neocortex
title_fullStr	Serotonin receptor 3A controls interneuron migration into the neocortex
title_full_unstemmed	Serotonin receptor 3A controls interneuron migration into the neocortex
title_sort	serotonin receptor 3a controls interneuron migration into the neocortex
description	Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic serotonin receptor 3A (5-HT3AR) is specifically expressed in CGE-derived migrating interneurons and upregulated while they invade the developing cortex. Functional investigations using calcium imaging, electrophysiological recordings and migration assays indicate that CGE-derived INs increase their response to 5-HT3AR activation during the late phase of cortical plate invasion. Using genetic loss-of-function approaches and in vivo grafts, we further demonstrate that the 5-HT3AR is cell autonomously required for the migration and proper positioning of reelin-expressing CGE-derived INs in the neocortex. Our findings reveal a requirement for a serotonin receptor in controlling the migration and laminar positioning of a specific subtype of cortical IN.
publisher	Nature Pub. Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263148/
_version_	1613166441050144768

Serotonin receptor 3A controls interneuron migration into the neocortex

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