Validation of a multiplex reverse transcription and pre-amplification method using TaqMan® MicroRNA assays
Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan® technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This meth...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244598/ |