Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance

Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance

Background: Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis. However, the low information density of DNA built from just...

Full description

Bibliographic Details
Main Authors: Merritt, Kristen K, Bradley, Kevin M, Hutter, Daniel, Matsuura, Mariko F, Rowold, Diane J, Benner, Steven A
Format: Online
Language:English
Published: Beilstein-Institut 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222377/
id pubmed-4222377
recordtype oai_dc
spelling pubmed-42223772014-11-07 Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance Merritt, Kristen K Bradley, Kevin M Hutter, Daniel Matsuura, Mariko F Rowold, Diane J Benner, Steven A Full Research Paper Background: Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis. However, the low information density of DNA built from just four nucleotide “letters”, the presence of strong (G:C) and weak (A:T) nucleobase pairs, the non-canonical folded structures that compete with Watson–Crick pairing, and other features intrinsic to natural DNA, generally prevent the autonomous assembly of short single-stranded oligonucleotides greater than a dozen or so. Beilstein-Institut 2014-10-09 /pmc/articles/PMC4222377/ /pubmed/25383105 http://dx.doi.org/10.3762/bjoc.10.245 Text en Copyright © 2014, Merritt et al; licensee Beilstein-Institut. http://www.beilstein-journals.org/bjoc This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (http://www.beilstein-journals.org/bjoc)
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Merritt, Kristen K
Bradley, Kevin M
Hutter, Daniel
Matsuura, Mariko F
Rowold, Diane J
Benner, Steven A
spellingShingle Merritt, Kristen K
Bradley, Kevin M
Hutter, Daniel
Matsuura, Mariko F
Rowold, Diane J
Benner, Steven A
Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
author_facet Merritt, Kristen K
Bradley, Kevin M
Hutter, Daniel
Matsuura, Mariko F
Rowold, Diane J
Benner, Steven A
author_sort Merritt, Kristen K
title Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
title_short Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
title_full Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
title_fullStr Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
title_full_unstemmed Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
title_sort autonomous assembly of synthetic oligonucleotides built from an expanded dna alphabet. total synthesis of a gene encoding kanamycin resistance
description Background: Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis. However, the low information density of DNA built from just four nucleotide “letters”, the presence of strong (G:C) and weak (A:T) nucleobase pairs, the non-canonical folded structures that compete with Watson–Crick pairing, and other features intrinsic to natural DNA, generally prevent the autonomous assembly of short single-stranded oligonucleotides greater than a dozen or so.
publisher Beilstein-Institut
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222377/
_version_ 1613153033017884672

Similar Items