Collective and Individual Migration following the Epithelial-Mesenchymal Transition
During cancer progression, malignant cells in the tumour invade surrounding tissues. This transformation of adherent cells to a motile phenotype has been associated with the epithelial mesenchymal transition (EMT). Here, we show that EMT-activated cells migrate through micropillar arrays as a collec...
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| Format: | Online |
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2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209198/ |
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pubmed-4209198 |
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pubmed-42091982015-05-01 Collective and Individual Migration following the Epithelial-Mesenchymal Transition Wong, Ian Y. Javaid, Sarah Wong, Elisabeth A. Perk, Sinem Haber, Daniel A. Toner, Mehmet Irimia, Daniel Article During cancer progression, malignant cells in the tumour invade surrounding tissues. This transformation of adherent cells to a motile phenotype has been associated with the epithelial mesenchymal transition (EMT). Here, we show that EMT-activated cells migrate through micropillar arrays as a collectively advancing front that scatters individual cells. Individual cells with few neighbours dispersed with fast, straight trajectories, whereas cells that encountered many neighbours migrated collectively with epithelial biomarkers. We modelled these emergent dynamics using a physical analogy to solidification phase transitions in binary mixtures, and validated it using drug perturbations, which revealed that individually migrating cells exhibit diminished chemosensitivity. Our measurements also indicate a degree of phenotypic plasticity as cells interconvert between individual and collective migration. The study of multicellular behaviours with single-cell resolution should enable further quantitative insights into heterogeneous tumour invasion. 2014-08-17 2014-11 /pmc/articles/PMC4209198/ /pubmed/25129619 http://dx.doi.org/10.1038/nmat4062 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wong, Ian Y. Javaid, Sarah Wong, Elisabeth A. Perk, Sinem Haber, Daniel A. Toner, Mehmet Irimia, Daniel |
| spellingShingle |
Wong, Ian Y. Javaid, Sarah Wong, Elisabeth A. Perk, Sinem Haber, Daniel A. Toner, Mehmet Irimia, Daniel Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| author_facet |
Wong, Ian Y. Javaid, Sarah Wong, Elisabeth A. Perk, Sinem Haber, Daniel A. Toner, Mehmet Irimia, Daniel |
| author_sort |
Wong, Ian Y. |
| title |
Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| title_short |
Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| title_full |
Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| title_fullStr |
Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| title_full_unstemmed |
Collective and Individual Migration following the Epithelial-Mesenchymal Transition |
| title_sort |
collective and individual migration following the epithelial-mesenchymal transition |
| description |
During cancer progression, malignant cells in the tumour invade surrounding tissues. This transformation of adherent cells to a motile phenotype has been associated with the epithelial mesenchymal transition (EMT). Here, we show that EMT-activated cells migrate through micropillar arrays as a collectively advancing front that scatters individual cells. Individual cells with few neighbours dispersed with fast, straight trajectories, whereas cells that encountered many neighbours migrated collectively with epithelial biomarkers. We modelled these emergent dynamics using a physical analogy to solidification phase transitions in binary mixtures, and validated it using drug perturbations, which revealed that individually migrating cells exhibit diminished chemosensitivity. Our measurements also indicate a degree of phenotypic plasticity as cells interconvert between individual and collective migration. The study of multicellular behaviours with single-cell resolution should enable further quantitative insights into heterogeneous tumour invasion. |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209198/ |
| _version_ |
1613148721534468096 |