Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development

Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) mo...

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Main Authors:	Chen, Xuemei, Huang, Huizhe, Wang, Hua, Guo, Fengjin, Du, Xiaogang, Ma, Linqiang, Zhao, Liang, Pan, Zhuma, Gui, Haibo, Yuan, Taixian, Liu, Xin, Song, Lin, Wang, Yiquan, He, Junling, Lei, Han, Gao, Rui
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147360/

id	pubmed-4147360
recordtype	oai_dc
spelling	pubmed-41473602014-09-07 Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development Chen, Xuemei Huang, Huizhe Wang, Hua Guo, Fengjin Du, Xiaogang Ma, Linqiang Zhao, Liang Pan, Zhuma Gui, Haibo Yuan, Taixian Liu, Xin Song, Lin Wang, Yiquan He, Junling Lei, Han Gao, Rui Research Article Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects. Furthermore, we demonstrate that the phenotypes caused by the knockdown of Pax1b in zebrafish could not be phenocopied by pax9 MO and could not be rescued by either Pax1a or Pax9 overexpression. We further find that Pax1b affects the expression of col2a1, Uncx4.1, Noggin3, and aggrecan, confirming the role of Pax1b in chondrocyte differentiation and bone maturation. Moreover, we identify an interaction between PAX1 and FOXO1 and find that the interaction was enhanced under hypoxia stress. Together, this evidence for cell death caused by pax1b knockdown provides new insight into the role of the Pax protein family in cell fate determination and tissue specification. Hindawi Publishing Corporation 2014 2014-08-13 /pmc/articles/PMC4147360/ /pubmed/25197636 http://dx.doi.org/10.1155/2014/309385 Text en Copyright © 2014 Xuemei Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chen, Xuemei Huang, Huizhe Wang, Hua Guo, Fengjin Du, Xiaogang Ma, Linqiang Zhao, Liang Pan, Zhuma Gui, Haibo Yuan, Taixian Liu, Xin Song, Lin Wang, Yiquan He, Junling Lei, Han Gao, Rui
spellingShingle	Chen, Xuemei Huang, Huizhe Wang, Hua Guo, Fengjin Du, Xiaogang Ma, Linqiang Zhao, Liang Pan, Zhuma Gui, Haibo Yuan, Taixian Liu, Xin Song, Lin Wang, Yiquan He, Junling Lei, Han Gao, Rui Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
author_facet	Chen, Xuemei Huang, Huizhe Wang, Hua Guo, Fengjin Du, Xiaogang Ma, Linqiang Zhao, Liang Pan, Zhuma Gui, Haibo Yuan, Taixian Liu, Xin Song, Lin Wang, Yiquan He, Junling Lei, Han Gao, Rui
author_sort	Chen, Xuemei
title	Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
title_short	Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
title_full	Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
title_fullStr	Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
title_full_unstemmed	Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
title_sort	characterization of zebrafish pax1b and pax9 in fin bud development
description	Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects. Furthermore, we demonstrate that the phenotypes caused by the knockdown of Pax1b in zebrafish could not be phenocopied by pax9 MO and could not be rescued by either Pax1a or Pax9 overexpression. We further find that Pax1b affects the expression of col2a1, Uncx4.1, Noggin3, and aggrecan, confirming the role of Pax1b in chondrocyte differentiation and bone maturation. Moreover, we identify an interaction between PAX1 and FOXO1 and find that the interaction was enhanced under hypoxia stress. Together, this evidence for cell death caused by pax1b knockdown provides new insight into the role of the Pax protein family in cell fate determination and tissue specification.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147360/
_version_	1613128588080447488

Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development

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