Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons

The phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway is considered important for cell survival and has been shown to mediate various anti-apoptotic biological effects. This study explored the role of the Toll-like receptor 4 (TLR4)-mediated PI3K/AKT-glycogen syn-thase ki...

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Main Authors:	He, Yue, Zhou, Ailing, Jiang, Wei
Format:	Online
Language:	English
Published:	Medknow Publications & Media Pvt Ltd 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145995/

id	pubmed-4145995
recordtype	oai_dc
spelling	pubmed-41459952014-09-09 Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons He, Yue Zhou, Ailing Jiang, Wei Technique and Method Article: Basic Research in Neural Regeneration The phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway is considered important for cell survival and has been shown to mediate various anti-apoptotic biological effects. This study explored the role of the Toll-like receptor 4 (TLR4)-mediated PI3K/AKT-glycogen syn-thase kinase 3β (GSK-3β) signaling pathways in lipopolysaccharide-induced apoptosis in a primary culture of hippocampal neurons. Results demonstrated that the apoptotic ratio of hippocampal neurons stimulated by lipopolysaccharide was significantly higher compared with the control group. Both the expression of P-AKTSer473 and P-GSK-3βSer9 in hippocampal neurons stimulated by lipopo-polysaccharide decreased compared with the control, while the level of active Caspase-3 and the ratio of Bax/Bcl-2 were significantly increased. The level of active Caspase-3 and the ratio of Bax/Bcl-2 in hippocampal neurons treated with TLR4 antibody or the GSK-3β inhibitor, LiCl, creased before intervention with lipopolysaccharide, but increased after treatment with the AKT hibitor, LY294002. These findings suggest that the TLR4-PI3K/AKT-GSK3β signaling pathway may be involved in lipopolysaccharide-induced apoptosis of hippocampal neurons. Medknow Publications & Media Pvt Ltd 2013-10-15 /pmc/articles/PMC4145995/ /pubmed/25206585 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.29.006 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	He, Yue Zhou, Ailing Jiang, Wei
spellingShingle	He, Yue Zhou, Ailing Jiang, Wei Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
author_facet	He, Yue Zhou, Ailing Jiang, Wei
author_sort	He, Yue
title	Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
title_short	Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
title_full	Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
title_fullStr	Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
title_full_unstemmed	Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
title_sort	toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons
description	The phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway is considered important for cell survival and has been shown to mediate various anti-apoptotic biological effects. This study explored the role of the Toll-like receptor 4 (TLR4)-mediated PI3K/AKT-glycogen syn-thase kinase 3β (GSK-3β) signaling pathways in lipopolysaccharide-induced apoptosis in a primary culture of hippocampal neurons. Results demonstrated that the apoptotic ratio of hippocampal neurons stimulated by lipopolysaccharide was significantly higher compared with the control group. Both the expression of P-AKTSer473 and P-GSK-3βSer9 in hippocampal neurons stimulated by lipopo-polysaccharide decreased compared with the control, while the level of active Caspase-3 and the ratio of Bax/Bcl-2 were significantly increased. The level of active Caspase-3 and the ratio of Bax/Bcl-2 in hippocampal neurons treated with TLR4 antibody or the GSK-3β inhibitor, LiCl, creased before intervention with lipopolysaccharide, but increased after treatment with the AKT hibitor, LY294002. These findings suggest that the TLR4-PI3K/AKT-GSK3β signaling pathway may be involved in lipopolysaccharide-induced apoptosis of hippocampal neurons.
publisher	Medknow Publications & Media Pvt Ltd
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145995/
_version_	1613128165777997824

Toll-like receptor 4-mediated signaling participates in apoptosis of hippocampal neurons

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