The Clinicopathological Significance of miR-133a in Colorectal Cancer
This study determined the expression of microRNA-133a (MiR-133a) in colorectal cancer (CRC) and adjacent normal mucosa samples and evaluated its clinicopathological role in CRC. The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101241/ |
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pubmed-4101241 |
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pubmed-41012412014-08-07 The Clinicopathological Significance of miR-133a in Colorectal Cancer Wan, Timothy Ming-Hun Lam, Colin Siu-Chi Ng, Lui Chow, Ariel Ka-Man Wong, Sunny Kit-Man Li, Hung-Sing Man, Johnny Hon-Wai Lo, Oswens Siu-Hung Foo, Dominic Cheung, Alvin Yau, Thomas Poon, Jensen Tung-Chung Poon, Ronnie Tung-Ping Law, Wai-Lun Pang, Roberta Wen-Chi Research Article This study determined the expression of microRNA-133a (MiR-133a) in colorectal cancer (CRC) and adjacent normal mucosa samples and evaluated its clinicopathological role in CRC. The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and correlated with patient's clinicopathological data by statistical analysis. Endogenous expression levels of several potential target genes were determined by qRT-PCR and correlated using Pearson's method. MiR-133a was downregulated in 83.2% of tumors compared to normal mucosal tissue. Higher miR-133a expression in tumor tissues was associated with development of distant metastasis, advanced Dukes and TNM staging, and poor survival. The unfavorable prognosis of higher miR-133a expression was accompanied by dysregulation of potential miR-133a target genes, LIM and SH3 domain protein 1 (LASP1), Caveolin-1 (CAV1), and Fascin-1 (FSCN1). LASP1 was found to possess a negative correlation (γ = −0.23), whereas CAV1 exhibited a significant positive correlation (γ = 0.27), and a stronger correlation was found in patients who developed distant metastases (γ = 0.42). In addition, a negative correlation of FSCN1 was only found in nonmetastatic patients. In conclusion, miR-133a was downregulated in CRC tissues, but its higher expression correlated with adverse clinical characteristics and poor prognosis. Hindawi Publishing Corporation 2014 2014-07-01 /pmc/articles/PMC4101241/ /pubmed/25104873 http://dx.doi.org/10.1155/2014/919283 Text en Copyright © 2014 Timothy Ming-Hun Wan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wan, Timothy Ming-Hun Lam, Colin Siu-Chi Ng, Lui Chow, Ariel Ka-Man Wong, Sunny Kit-Man Li, Hung-Sing Man, Johnny Hon-Wai Lo, Oswens Siu-Hung Foo, Dominic Cheung, Alvin Yau, Thomas Poon, Jensen Tung-Chung Poon, Ronnie Tung-Ping Law, Wai-Lun Pang, Roberta Wen-Chi |
| spellingShingle |
Wan, Timothy Ming-Hun Lam, Colin Siu-Chi Ng, Lui Chow, Ariel Ka-Man Wong, Sunny Kit-Man Li, Hung-Sing Man, Johnny Hon-Wai Lo, Oswens Siu-Hung Foo, Dominic Cheung, Alvin Yau, Thomas Poon, Jensen Tung-Chung Poon, Ronnie Tung-Ping Law, Wai-Lun Pang, Roberta Wen-Chi The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| author_facet |
Wan, Timothy Ming-Hun Lam, Colin Siu-Chi Ng, Lui Chow, Ariel Ka-Man Wong, Sunny Kit-Man Li, Hung-Sing Man, Johnny Hon-Wai Lo, Oswens Siu-Hung Foo, Dominic Cheung, Alvin Yau, Thomas Poon, Jensen Tung-Chung Poon, Ronnie Tung-Ping Law, Wai-Lun Pang, Roberta Wen-Chi |
| author_sort |
Wan, Timothy Ming-Hun |
| title |
The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| title_short |
The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| title_full |
The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| title_fullStr |
The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| title_full_unstemmed |
The Clinicopathological Significance of miR-133a in Colorectal Cancer |
| title_sort |
clinicopathological significance of mir-133a in colorectal cancer |
| description |
This study determined the expression of microRNA-133a (MiR-133a) in colorectal cancer (CRC) and adjacent normal mucosa samples and evaluated its clinicopathological role in CRC. The expression of miR-133a in 125 pairs of tissue samples was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and correlated with patient's clinicopathological data by statistical analysis. Endogenous expression levels of several potential target genes were determined by qRT-PCR and correlated using Pearson's method. MiR-133a was downregulated in 83.2% of tumors compared to normal mucosal tissue. Higher miR-133a expression in tumor tissues was associated with development of distant metastasis, advanced Dukes and TNM staging, and poor survival. The unfavorable prognosis of higher miR-133a expression was accompanied by dysregulation of potential miR-133a target genes, LIM and SH3 domain protein 1 (LASP1), Caveolin-1 (CAV1), and Fascin-1 (FSCN1). LASP1 was found to possess a negative correlation (γ = −0.23), whereas CAV1 exhibited a significant positive correlation (γ = 0.27), and a stronger correlation was found in patients who developed distant metastases (γ = 0.42). In addition, a negative correlation of FSCN1 was only found in nonmetastatic patients. In conclusion, miR-133a was downregulated in CRC tissues, but its higher expression correlated with adverse clinical characteristics and poor prognosis. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101241/ |
| _version_ |
1613114872083513344 |