Theoretical and experimental assessment of degenerate primer tagging in ultra-deep applications of next-generation sequencing

Theoretical and experimental assessment of degenerate primer tagging in ultra-deep applications of next-generation sequencing

Primer IDs (pIDs) are random oligonucleotide tags used in next-generation sequencing to identify sequences that originate from the same template. These tags are produced by degenerate primers during the reverse transcription of RNA molecules into cDNA. The use of pIDs helps to track the number of RN...

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Bibliographic Details
Main Authors: Liang, Richard H., Mo, Theresa, Dong, Winnie, Lee, Guinevere Q., Swenson, Luke C., McCloskey, Rosemary M., Woods, Conan K., Brumme, Chanson J., Ho, Cynthia K.Y., Schinkel, Janke, Joy, Jeffrey B., Harrigan, P. Richard, Poon, Art F.Y.
Format: Online
Language:English
Published: Oxford University Press 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081055/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081055/

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