Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms

We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approach...

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Bibliographic Details
Main Authors: Patro, Rob, Mount, Stephen M., Kingsford, Carl
Format: Online
Language:English
Published: 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4077321/