Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells

Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells

SUMOylation is a dynamic and reversible process. Several SUMO-specific proteases (SENPs) that remove SUMO from substrates have been shown to be amplified in a subset of cancers. SENP5 is required for cell division, as well as maintaining mitochondrial morphology and function. SENP5 has been reported...

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Main Authors: WANG, KUN, ZHANG, XIN-CHAO
Format: Online
Language:English
Published: D.A. Spandidos 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043617/
id pubmed-4043617
recordtype oai_dc
spelling pubmed-40436172014-06-12 Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells WANG, KUN ZHANG, XIN-CHAO Articles SUMOylation is a dynamic and reversible process. Several SUMO-specific proteases (SENPs) that remove SUMO from substrates have been shown to be amplified in a subset of cancers. SENP5 is required for cell division, as well as maintaining mitochondrial morphology and function. SENP5 has been reported to be predominantly localized to the cytoplasm of oral squamous cell carcinoma (OSCC) and is associated with the differentiation of OSCC. Western blot was used to detect the protein expression of SENP5 in osteosarcoma cells and tissue. Lentivirus-mediated siRNA was used to silence the expression of SENP5. Cell cycle distribution was determined by FACS analysis. The present study showed that SENP5 is overexpressed in osteosarcoma cells. In addition, lentivirus-mediated small interfering RNA (siRNA) of SENP5 significantly inhibited cell growth and induced apoptosis in osteosarcoma cells. SENP5 inhibition suppressed the growth and colony formation capacity of two osteosarcoma cell lines, U2OS and Saos-2. Silencing the expression of SENP5 in serum-starved U2OS and Saos-2 cells induced an increase in caspase-3/-7 activity and a decrease in cyclin B1 expression. These observations indicate that SENP5 is required for cell growth and apoptosis and may therefore be a promising drug target for antiosteosarcoma treatment. D.A. Spandidos 2014-06 2014-03-28 /pmc/articles/PMC4043617/ /pubmed/24926368 http://dx.doi.org/10.3892/etm.2014.1644 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author WANG, KUN
ZHANG, XIN-CHAO
spellingShingle WANG, KUN
ZHANG, XIN-CHAO
Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
author_facet WANG, KUN
ZHANG, XIN-CHAO
author_sort WANG, KUN
title Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
title_short Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
title_full Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
title_fullStr Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
title_full_unstemmed Inhibition of SENP5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
title_sort inhibition of senp5 suppresses cell growth and promotes apoptosis in osteosarcoma cells
description SUMOylation is a dynamic and reversible process. Several SUMO-specific proteases (SENPs) that remove SUMO from substrates have been shown to be amplified in a subset of cancers. SENP5 is required for cell division, as well as maintaining mitochondrial morphology and function. SENP5 has been reported to be predominantly localized to the cytoplasm of oral squamous cell carcinoma (OSCC) and is associated with the differentiation of OSCC. Western blot was used to detect the protein expression of SENP5 in osteosarcoma cells and tissue. Lentivirus-mediated siRNA was used to silence the expression of SENP5. Cell cycle distribution was determined by FACS analysis. The present study showed that SENP5 is overexpressed in osteosarcoma cells. In addition, lentivirus-mediated small interfering RNA (siRNA) of SENP5 significantly inhibited cell growth and induced apoptosis in osteosarcoma cells. SENP5 inhibition suppressed the growth and colony formation capacity of two osteosarcoma cell lines, U2OS and Saos-2. Silencing the expression of SENP5 in serum-starved U2OS and Saos-2 cells induced an increase in caspase-3/-7 activity and a decrease in cyclin B1 expression. These observations indicate that SENP5 is required for cell growth and apoptosis and may therefore be a promising drug target for antiosteosarcoma treatment.
publisher D.A. Spandidos
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043617/
_version_ 1612096026225672192

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