Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans
C. elegans chromosomes contain specialized regions called pairing centers (PCs) that mediate homologous pairing and synapsis during meiosis. Four related proteins, ZIM-1, -2, -3, and HIM-8, associate with these sites and are required for their essential functions. Here we show that short sequence el...
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| Format: | Online |
| Language: | English |
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2009
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001799/ |
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pubmed-40017992014-04-28 Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans Phillips, Carolyn M. Meng, Xiangdong Zhang, Lei Chretien, Jacqueline H. Urnov, Fyodor D. Dernburg, Abby F. Article C. elegans chromosomes contain specialized regions called pairing centers (PCs) that mediate homologous pairing and synapsis during meiosis. Four related proteins, ZIM-1, -2, -3, and HIM-8, associate with these sites and are required for their essential functions. Here we show that short sequence elements enriched in the corresponding chromosome regions selectively recruit these proteins in vivo. In vitro analysis using SELEX indicates that each protein’s binding specificity arises from a combination of two zinc fingers and an adjacent domain. Insertion of a cluster of recruiting motifs into a chromosome lacking its endogenous PC is sufficient to restore homologous pairing, synapsis, crossover recombination, and segregation. These findings help to illuminate how chromosome sites mediate essential aspects of meiotic chromosome dynamics. 2009-07-20 2009-08 /pmc/articles/PMC4001799/ /pubmed/19620970 http://dx.doi.org/10.1038/ncb1904 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Phillips, Carolyn M. Meng, Xiangdong Zhang, Lei Chretien, Jacqueline H. Urnov, Fyodor D. Dernburg, Abby F. |
| spellingShingle |
Phillips, Carolyn M. Meng, Xiangdong Zhang, Lei Chretien, Jacqueline H. Urnov, Fyodor D. Dernburg, Abby F. Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| author_facet |
Phillips, Carolyn M. Meng, Xiangdong Zhang, Lei Chretien, Jacqueline H. Urnov, Fyodor D. Dernburg, Abby F. |
| author_sort |
Phillips, Carolyn M. |
| title |
Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| title_short |
Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| title_full |
Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| title_fullStr |
Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| title_full_unstemmed |
Identification of Chromosome Sequence Motifs That Mediate Meiotic Pairing and Synapsis in C. elegans |
| title_sort |
identification of chromosome sequence motifs that mediate meiotic pairing and synapsis in c. elegans |
| description |
C. elegans chromosomes contain specialized regions called pairing centers (PCs) that mediate homologous pairing and synapsis during meiosis. Four related proteins, ZIM-1, -2, -3, and HIM-8, associate with these sites and are required for their essential functions. Here we show that short sequence elements enriched in the corresponding chromosome regions selectively recruit these proteins in vivo. In vitro analysis using SELEX indicates that each protein’s binding specificity arises from a combination of two zinc fingers and an adjacent domain. Insertion of a cluster of recruiting motifs into a chromosome lacking its endogenous PC is sufficient to restore homologous pairing, synapsis, crossover recombination, and segregation. These findings help to illuminate how chromosome sites mediate essential aspects of meiotic chromosome dynamics. |
| publishDate |
2009 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001799/ |
| _version_ |
1612083031047143424 |